JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB282309

人Interferon omega ELISA试剂盒

Human Interferon omega ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Interferon omega ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Interferon omega with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Interferon omega-1, Interferon alpha-II-1, IFNW1

2 Images
Sandwich ELISA - Human Interferon omega ELISA Kit (AB282309)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon omega ELISA Kit (AB282309)

Interpolated concentrations of spike Interferon omega in human serum, plasma, and cell culture media samples.

The concentrations of Interferon omega were measured in duplicates, interpolated from the Interferon omega standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 50% and cell culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon omega concentration was determined to be 591 pg/mL in serum, 595 pg/mL in plasma (citrate), 298 pg/mL in plasma (EDTA), 587 pg/mL in plasma (heparin), and 625 pg/ml in cell culture media.

Sandwich ELISA - Human Interferon omega ELISA Kit (AB282309)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon omega ELISA Kit (AB282309)

Example of human Interferon omega standard curve in Sample Diluent NS.

The Interferon omega standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 1.1 pg/mL

范围

10.94 - 700 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

搭配使用产品

Assay Kits

AB218268

HIV1 p24 ELISA Kit

SimpleStep

Sandwich ELISA - HIV1 p24 ELISA Kit (AB218268)

  • 0
  • 0
Proteins & Peptides

AB276259

Recombinant human IFNW1 protein (His tag)

Functional Studies - Recombinant human IFNW1 protein (His tag) (AB276259)

  • 0
  • 0
Proteins & Peptides

AB43037

Recombinant HIV1 p24 protein

SDS-PAGE - Recombinant HIV1 p24 protein (AB43037)

  • 0
  • 0
Primary Antibodies

AB32352

Anti-HIV1 p24 antibody [P131]

RabMAb|Recombinant

Western blot - Anti-HIV1 p24 antibody [P131] (AB32352)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human Interferon omega ELISA kit (ab282309) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Interferon omega protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Interferon omega with 1.1 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interferon omega is a type I interferon. It is a secreted monomeric glycoprotein distantly related in structure to Interferon alpha and Interferon beta. Interferon omega is expressed in and secreted from virus-infected leukocytes. Biological activity of Interferon omega, Interferon alpha and Interferon beta is mediated by Type I Interferon Receptor Complex.

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]
style
left-column

回收率

[ { "sample": "Serum", "range": "81 - 83 %", "average": "= 82" }, { "sample": "EDTA Plasma", "range": "90 - 91 %", "average": "= 91" }, { "sample": "Cell culture media", "range": "90 - 96 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "81 - 84 %", "average": "= 82" }, { "sample": "Citrate plasma", "range": "81 - 81 %", "average": "= 81" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282309-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB282309-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB282309-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282309-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282309-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282309-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282309-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282309-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Interferon omega Lyophilized Recombinant Protein", "number":"AB282309-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon omega Capture Antibody", "number":"AB282309-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon omega Detector Antibody", "number":"AB282309-CMP03", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column
style
left-column

产品实验方案

style
left-column

靶点信息

See full target information IFNW1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com