AB173193

人Interferon gamma Receptor 1 ELISA试剂盒(CD119)

Human Interferon gamma Receptor 1 ELISA Kit (CD119)

SimpleStep
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Human Interferon gamma Receptor 1 ELISA Kit (CD119) is a single-wash 90-min Simplestep used to quantify Human Interferon gamma Receptor 1 (CD119) with a sensitivity of 140 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

查看别名

CD119, Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, CDw119, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha, IFNGR1

4 Images

ELISA - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (AB173193)

ELISA

Supplier Data

ELISA - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (AB173193)

Example IFNGR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed. A new standard curve must be generated for each assay performed.

ELISA

Supplier Data

ELISA - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (AB173193)

Quantification of IFNGR1 expression in different mammalian cell lines. Interpolated values of IFNGR1 are plotted for the indicated cell lines based on a lysate concentration of 100 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (AB173193)

Representative image of Human Interferon gamma Receptor 1 ELISA Kit (CD119) (ab173193)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Interferon gamma Receptor 1 (CD119) Capture Antibody

- 10X Human Interferon gamma Receptor 1 (CD119) Detector Antibody

- Human Interferon gamma Receptor 1 (CD119) Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5B

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

ELISA

Supplier Data

ELISA - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (AB173193)

ELISA typical data.

Titration of MCF7 cell lysate within the working range of the assay. Background subtracted data from triplicate measurements are plotted.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts, Suspension cells, Tissue Homogenate, Adherent cells, Cell Lysate

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 140 pg/mL

范围

0.14 - 100 ng/mL

检测时间

1h 30m

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Interferon-gamma Receptor 1 (CD119) ELISA kit (ab173194) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon-gamma Receptor 1 protein in cell lysates and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Interferon-gamma Receptor 1 with 140 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

Interferon gamma receptor 1 (IFNGR1) is a heterodimeric receptor that binds one interferon gamma dimer as a ligand. The receptor is a single-pass type I membrane protein present on the plasma membrane. A genetic variation in the IFNGR1 gene is associated with susceptibility to Helicobacter pylori infection. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "MCF7 Lysate", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "MCF7 Lysate", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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回收率

[ { "sample": "Cell culture media", "range": "80 - 94 %", "average": "= 84" }, { "sample": "Plasma", "range": "73 - 85 %", "average": "= 78" }, { "sample": "Serum", "range": "72 - 87 %", "average": "= 82" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab173193-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB173193-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB173193-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB173193-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB173193-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB173193-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB173193-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5B", "number":"AB173193-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IFNGR1 Capture Antibody", "number":"AB173193-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB173193-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB173193-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IFNGR1 Lyophilized Recombinant Protein", "number":"AB173193-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IFNGR1 Detector Antibody", "number":"AB173193-CMP10", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The IFNGR1 protein also known as Interferon Gamma Receptor 1 is an integral component of the cell surface receptor for interferon-gamma (IFN-γ). It possesses a mass of approximately 53 kDa. IFNGR1 is expressed in various cell types including immune cells such as T cells and macrophages and is also present in other cell lines like HEK 293. This receptor binds to its specific ligand IFN-γ initiating a series of intracellular events that are essential for immune response modulation.

Biological function summary

IFNGR1 functions as part of the interferon-gamma receptor complex working alongside the IFNGR2 subunit. This interaction with IFNGR2 is important for the receptor to properly transmit signals inside the cell. The binding of interferon-gamma to this receptor complex activates the JAK-STAT signaling pathway promoting the transcription of genes that enhance the antimicrobial activity of immune cells and regulate cellular immunity.

Pathways

The IFNGR1 protein plays an important role in the JAK-STAT signaling pathway which is critical for mediating responses to interferon-gamma. Upon activation by IFN-γ IFNGR1 recruits JAK kinases leading to the phosphorylation of STAT1 a significant transcription factor. STAT1 then dimerizes and translocates to the nucleus where it induces the expression of genes involved in immune defense and inflammation. This process is vital for the body's ability to handle infections and other immunological challenges.

IFNGR1 is associated with susceptibility to infections and certain immune-related disorders. Mutations or deficiencies in IFNGR1 can lead to Mendelian Susceptibility to Mycobacterial Disease (MSMD) where individuals show increased vulnerability to mycobacterial infections. Additionally improper signaling through IFNGR1 is linked to chronic granulomatous disease which can involve defective functioning of the NADPH oxidase complex. Understanding IFNGR1's function and interactions is important for exploring new therapeutic strategies for these disorders.

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产品实验方案

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靶点信息

Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 20015550). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor (PubMed : 10986460, PubMed : 2971451, PubMed : 7615558, PubMed : 7617032, PubMed : 7673114). Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription (PubMed : 28883123). STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (By similarity).

See full target information IFNGR1

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websiteProtocolBooklet

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人Interferon gamma Receptor 1 ELISA试剂盒(CD119)

Human Interferon gamma Receptor 1 ELISA Kit (CD119)

ELISA - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (AB173193)

ELISA - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (AB173193)

Sandwich ELISA - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (AB173193)

ELISA - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (AB173193)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台