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AB327293

Human Interferon alpha 2 ELISA Kit, Chemiluminescent

Human Interferon alpha 2 ELISA Kit, Chemiluminescent

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Human Interferon alpha 2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Interferon alpha 2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 32.92 - 72000 pg/ml with a sensitivity of 13.3 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

查看别名

IFNA2A, IFNA2B, IFNA2C, IFNA2, Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A

3 Images
Sandwich ELISA - Human Interferon alpha 2 ELISA Kit, Chemiluminescent (AB327293)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 2 ELISA Kit, Chemiluminescent (AB327293)

Interpolated concentrations of spiked Interferon Alpha 2 in human serum plasma and culture media samples.

The concentrations of Interferon Alpha 2 were measured in duplicates interpolated from the Interferon Alpha 2 standard curves.

Sandwich ELISA - Human Interferon alpha 2 ELISA Kit, Chemiluminescent (AB327293)
  • sELISA

Lab

Sandwich ELISA - Human Interferon alpha 2 ELISA Kit, Chemiluminescent (AB327293)

Standard curve comparison between the Chemiluminescent Human Interferon alpha 2 SimpleStep ELISA and colorimetric Human Interferon alpha 2 SimpleStep ELISA (ab233622) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Interferon alpha 2 ELISA Kit, Chemiluminescent (AB327293)
  • sELISA

Lab

Sandwich ELISA - Human Interferon alpha 2 ELISA Kit, Chemiluminescent (AB327293)

Dynamic range comparison between the Chemiluminescent Human Interferon alpha 2 SimpleStep ELISA and colorimetric Human Interferon alpha 2 SimpleStep ELISA (ab233622). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

关键信息

检测方法

Luminescent

样品类型

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 13.3 pg/mL

范围

32.92 - 72000 pg/mL

检测时间

55m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Interferon alpha 2 ELISA Kit, Chemiluminescent (ab327293) is a rapid single-wash sandwich 55-min ELISA to measure Human Interferon alpha 2 in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 13.3 pg/mL.

How the assay works

Human Interferon alpha 2 ELISA Kit, Chemiluminescent (ab327293) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Interferon alpha 2 (ab327293) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IFNA2 antibody [EPR19074-207] - BSA and Aside free (Capture) ab244841
- Anti-IFNA2 antibody [EPR19074-55] - BSA and Aside free (Detector) ab245007
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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" } ]
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回收率

[ { "sample": "Serum", "range": "99 - 109 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "80 - 93 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "94 - 108 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "102 - 103 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "100 - 116 %", "average": "= 106" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Produced by macrophages, IFN-alpha have antiviral activities.
See full target information IFNA2
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websiteProtocolBooklet
en
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