AB233622

人Interferon Alpha 2 ELISA试剂盒

Human Interferon Alpha 2 ELISA Kit

Recombinant
SimpleStep
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Human Interferon Alpha 2 ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human Interferon Alpha 2 with a sensitivity of 56.04 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab327293

查看别名

IFNA2A, IFNA2B, IFNA2C, IFNA2, Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A

2 Images

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

sELISA

Supplier Data

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

Interpolated concentrations of spiked Interferon Alpha 2 in human serum, plasma and culture media samples.

The concentrations of Interferon Alpha 2 were measured in duplicates, interpolated from the Interferon Alpha 2 standard curves.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

Example of human Interferon Alpha 2 standard curve in Sample Diluent NS.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma, Cell culture media

反应种属

Human

检测类型

Sandwich

Quantitative

灵敏度

= 56.04 pg/mL

范围

187.5 - 12000 pg/mL

检测时间

1h 30m

检测平台

Microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Interferon Alpha 2 ELISA Kit (ab233622) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon Alpha 2 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Interferon Alpha 2 with 56.04 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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回收率

[ { "sample": "Serum", "range": "99 - 109 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "80 - 93 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "94 - 108 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "102 - 103 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "100 - 116 %", "average": "= 106" } ]

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规格

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab233622-10x96Tests", "assetComponentDetails": [ { "size":"1 x 6000 µL", "name":"10X Human Interferon Alpha 2 Capture Antibody", "number":"AB233622-CMP02" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233622-CMP04" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB233622-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233622-CMP05" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB233622-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Human Interferon Alpha 2 Detector Antibody", "number":"AB233622-CMP03" }, { "size":"2 x 10 Vial", "name":"Human Interferon Alpha 2 Lyophilized Recombinant Protein", "number":"AB233622-CMP08" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB233622-CMP07" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB233622-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233622-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233622-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233622-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human Interferon Alpha 2 Capture Antibody", "number":"AB233622-CMP02" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233622-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB233622-CMP01" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233622-CMP04" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233622-CMP07" }, { "size":"2 x 1 Vial", "name":"Human Interferon Alpha 2 Lyophilized Recombinant Protein", "number":"AB233622-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human Interferon Alpha 2 Detector Antibody", "number":"AB233622-CMP03" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB233622-CMP09" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233622-CMP06" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab233622-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB233622-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB233622-CMP06" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233622-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB233622-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233622-CMP04" }, { "size":"2 x 10 Vial", "name":"Human Interferon Alpha 2 Lyophilized Recombinant Protein", "number":"AB233622-CMP08" }, { "size":"1 x 6000 µL", "name":"10X Human Interferon Alpha 2 Capture Antibody", "number":"AB233622-CMP02" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB233622-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233622-CMP05" }, { "size":"1 x 6000 µL", "name":"10X Human Interferon Alpha 2 Detector Antibody", "number":"AB233622-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233622-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB233622-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB233622-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233622-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233622-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233622-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233622-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233622-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Interferon Alpha 2 Lyophilized Recombinant Protein", "number":"AB233622-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon Alpha 2 Capture Antibody", "number":"AB233622-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon Alpha 2 Detector Antibody", "number":"AB233622-CMP03", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 2 also known as IFN alpha 2 or IFN alpha 2a/b is a type of cytokine that plays a critical role in the immune response. It is a smaller protein weighing approximately 19.5 kDa mainly expressed by leukocytes such as macrophages and dendritic cells. This protein exhibits antiviral antiproliferative and immunomodulatory activities by binding to specific receptors on target cells. Researchers often study interferon alpha through recombinant protein studies which can be simulated by companies like PeproTech. Additionally ELISA assays facilitate the quantitative measurement of interferon alpha levels in various biological samples.

Biological function summary

Interferon alpha 2 engages in complex signaling with other immune factors to establish an antiviral state in cells. This protein binds to the IFNAR1 and IFNAR2 receptor complex initiating a cascade that activates genes responsible for immune defense. By doing so it triggers the transcription of various antiviral proteins and enhances the activity of immune cells including natural killer cells and macrophages. Its function is also evaluated in reconstitution studies such as PeproTech's IL-2 reconstitution to investigate broader immune functions.

Pathways

Researchers observe interferon alpha 2 predominantly within the JAK-STAT signaling pathway. This pathway is vital in transmitting the extracellular signal through to the gene transcription process leading to an appropriate immune response. STAT proteins such as STAT1 and STAT2 form a transcription complex following activation by JAK kinases. Interferon alpha 2's interactions in the pathway exemplify how this cytokine links with other components for immune regulation coordinating with other proteins like ISGF3 to amplify antiviral responses.

Scientists associate interferon alpha 2 with chronic viral infections and certain cancers. For example its therapeutic application has been prominent in treating hepatitis C and some hematological malignancies. In viral infections interferon alpha inhibits viral replication and enhances antiviral immunity. In cancer it suppresses cell proliferation and regulates immune surveillance. The protein's levels and activity can correlate with pathological states influenced by other proteins such as IFN-lambda and TNF-alpha highlighting its role in disease mechanisms and therapeutic interventions.

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产品实验方案

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靶点信息

Produced by macrophages, IFN-alpha have antiviral activities.

See full target information IFNA2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:100894 PubMed36652906

2023

Inactive disease in patients with lupus is linked to autoantibodies to type I interferons that normalize blood IFNα and B cell subsets.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah F Bradford,Liis Haljasmägi,Madhvi Menon,Thomas C R McDonnell,Karita Särekannu,Martti Vanker,Pärt Peterson,Chris Wincup,Rym Abida,Raquel Fernandez Gonzalez,Vincent Bondet,Darragh Duffy,David A Isenberg,Kai Kisand,Claudia Mauri

View all publications

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websiteProtocolBooklet

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人Interferon Alpha 2 ELISA试剂盒

Human Interferon Alpha 2 ELISA Kit

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台

反应性数据

产品详情

精确度

回收率

规格

性能和储存信息

运输条件

推荐的短期储存条件

推荐的长期储存条件

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (1)

Abcam Product Promise

人Interferon Alpha 2 ELISA试剂盒

Human Interferon Alpha 2 ELISA Kit

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台

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反应性数据

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精确度

回收率

规格

性能和储存信息

运输条件

推荐的短期储存条件

推荐的长期储存条件

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (1)

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