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AB309428

人Integrin alpha 2 ELISA试剂盒

Human Integrin alpha 2 ELISA kit

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Human Integrin alpha 2 ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Human Integrin alpha 2 in Human in Biofluids, Cell Culture Media samples.

查看别名

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

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Sandwich ELISA - Human Integrin alpha 2 ELISA kit (AB309428)
  • sELISA

Supplier Data

Sandwich ELISA - Human Integrin alpha 2 ELISA kit (AB309428)

Example of standard curve using ab309428. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Serum, Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 0.1 ng/mL

范围

0.102 - 25 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Integrin alpha 2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Integrin alpha 2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Integrin alpha 2 coated on a 96-well plate
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回收率

[ { "sample": "Serum", "range": "105 - 131 %", "average": "= 120.6" }, { "sample": "Plasma", "range": "84 - 120 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "95 - 137 %", "average": "= 116.7" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309428-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309428-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ITGA2 Protein Standard", "number":"AB309428-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309428-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309428-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human ITGA2 Detection Antibody", "number":"AB309428-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ITGA2 Antibody-coated ELISA Plate", "number":"AB309428-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309428-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309428-CMP04", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.
Biological function summary

Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.

Pathways

Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.

Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.
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产品实验方案

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靶点信息

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human rotavirus A.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human echoviruses 1 and 8.
See full target information ITGA2
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB181548

Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (AB181548)

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