人Immunoglobulin E ELISA试剂盒(IgE) (ab178659)
Key features and details
- Sensitivity: 0.27 IU/ml
- Range: 0 IU/ml - 200 IU/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Immunoglobulin E ELISA试剂盒(IgE)
参阅全部 IgE 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 16 <=7.2% 批次间 样品 n Mean SD CV% Serum 16 <=7.6% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.27 IU/ml -
范围
0 IU/ml - 200 IU/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Immunogobulin E (IgE) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgE in serum and plasma.
The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-IgE antibody. Upon mixing monoclonal biotinylated antibody, and a serum containing the native antigen, reaction results between the native antigen and the antibody,forming an Antibody-Antigen complex. Simultaneously, the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody. After a suitable incubation period, the antibody-antigen bound fraction is separated from unbound antigen by decantation or aspiration. Another antibody (directed at a different epitope) labelled with an enzyme is added. Another interaction occurs to form an Enzyme labelled Antibody-Antigen-Biotinylated-Antibody complex on the surface of the wells. Excess enzyme is washed off via a wash step. A suitable substrate is added to produce colour measurable with the use of a microplate spectrophotometer. The enzyme activity on the well is directly proportional to the native free antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
Get higher sensitivity in only 90 minutes with Human IgE ELISA Kit (ab195216) from our SimpleStep ELISA® range.
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说明
IgE is an antibody isotype, found only in mammals. Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/mL, compared to 10 mg/mL for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from an allergy known as type 1 hypersensitivity.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 50X Washing Solution 1 x 20ml Cover Foil 1 unit IgE Biotin Conjugate 1 x 13ml IgE HRP Conjugate 1 x 13ml IgE Standard 0 - 0 IU/mL 1 x 1ml IgE Standard 1 - 5 IU/mL 1 x 1ml IgE Standard 2 - 25 IU/mL 1 x 1ml IgE Standard 3 - 50 IU/mL 1 x 1ml IgE Standard 4 - 150 IU/mL 1 x 1ml IgE Standard 5 - 400 IU/mL 1 x 1ml Stop Solution 1 x 15ml Streptavidin Coated Microplate (12 x 8 wells) 1 unit Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究领域
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相关性
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
细胞定位
Secreted -
别名
- Ig epsilon chain C region
- IgE
- IGHE
- immunoglobulin heavy constant epsilon
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数据库链接
- Entrez Gene: 3497 Human
- Omim: 147180 Human
- SwissProt: P01854 Human
相关产品
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SimpleStep ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178659于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab178659 被引用在 1 文献中.
- Saad EA et al. MUC7 VNTR polymorphism and association with bronchial asthma in Egyptian children. Sci Rep 12:18910 (2022). PubMed: 36344553