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AB242237

人IL-9 ELISA试剂盒

Human IL-9 ELISA Kit

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Human IL-9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-9 with a sensitivity of 2.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Interleukin-9, IL-9, Cytokine P40, T-cell growth factor P40, IL9

3 Images
Sandwich ELISA - Human IL-9 ELISA Kit (AB242237)
  • sELISA

Unknown

Sandwich ELISA - Human IL-9 ELISA Kit (AB242237)

Interpolated concentrations of native IL-9 in human HDLM-2 cell culture supernatant samples. The concentrations of IL-9 were measured in duplicates, interpolated from the IL-9 standard curves and corrected for sample dilution. Undiluted samples are as follows : HDLM-2 supernatant 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-9 concentration was determined to be 65.7 ng/ml in HDLM-2 supernatant.

Sandwich ELISA - Human IL-9 ELISA Kit (AB242237)
  • sELISA

Unknown

Sandwich ELISA - Human IL-9 ELISA Kit (AB242237)

Human IL-9 Standard Curve Comparison. Standard Curve comparison between human IL-9 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human IL-9 ELISA Kit (AB242237)
  • sELISA

Unknown

Sandwich ELISA - Human IL-9 ELISA Kit (AB242237)

Example of human IL-9 standard curve in Sample Diluent 75BS. The IL-9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 2.3 pg/mL

范围

9.38 - 600 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Primary Antibodies

AB227037

Anti-IL-9 antibody [EPR23484-151]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-IL-9 antibody [EPR23484-151] (AB227037)

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  • 0
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

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  • 4
Assay Kits

AB185986

Human IL-10 ELISA Kit (Interleukin-10)

KO Validated|Recombinant|SimpleStep

ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human IL-9 ELISA Kit (ab242237) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-9 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-9 with 2.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "96 - 119 %", "average": "= 110" }, { "sample": "Serum", "range": "92 - 111 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "97 - 111 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "99 - 105 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "95 - 109 %", "average": "= 101" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242237-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB242237-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB242237-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242237-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242237-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242237-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242237-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242237-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BS", "number":"AB242237-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-9 Capture Antibody", "number":"AB242237-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-9 Lyophilized recombinant Protein", "number":"AB242237-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-9 Detector Antibody", "number":"AB242237-CMP05", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-9 (IL-9) sometimes known as a T-cell growth factor is a cytokine with a molecular mass of approximately 14-19 kDa. It is primarily expressed by T helper cells particularly Th2 and Th9 cells. IL-9 comes from the IL-9 gene and plays a significant role in the immune system by affecting various cell types such as mast cells B cells and epithelial cells. Researchers first identified it in the late 1980s and it has continued to be an area of interest due to its diverse functions.
Biological function summary

IL-9 influences immune responses and is important for various processes including cell proliferation and survival. IL-9 activates mast cells which play role in allergy response. It also affects the growth and differentiation of T cells and enhances the function of dendritic cells. IL-9 operates individually and not as part of a larger complex but works closely with its receptor IL-9R to initiate downstream signaling events.

Pathways

Cytokine signaling pathways and JAK-STAT signaling pathways integrate IL-9's function in the immune system. IL-9 activates the JAK-STAT pathway particularly involving proteins like JAK1 and STATs to relay signals that promote immune cell growth and survival. This cytokine also interlinks with other cytokines such as IL-4 and IL-13 playing role in immune regulation and inflammatory responses.

IL-9 shows strong correlation with asthma and certain types of cancers particularly Hodgkin's lymphoma. In asthma overproduction of IL-9 can lead to increased mucus production and airway hyperresponsiveness. This relationship makes it a potential target for blocking therapies in asthma treatment. Higher levels of IL-9 correlate with poorer prognosis in some cancers indicating its potential role in tumor growth and immune evasion possibly interacting with IL-6 in such conditions.
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产品实验方案

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靶点信息

Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites (PubMed : 29742432). Affects intestinal epithelial permeability and adaptive immunity (PubMed : 29742432). In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells. Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG. Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs. Induction of differentiation genes seems to be mediated by STAT1 alone, while protection of cells from apoptosis depends on STAT3 and STAT5.
See full target information IL9
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websiteProtocolBooklet
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