人IL-8 ELISA试剂盒(without plates) (ab48481)
概述
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产品名称
人IL-8 ELISA试剂盒(without plates)
参阅全部 IL-8 试剂盒 -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 12.3 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
检测时间
2h 15m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-8 Human in vitro ELISA Set is designed for the quantitative measurement of IL-8 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The IL-8 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA set is intended to measure human IL-8 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Sensitivity: < 29 pg / ml
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-IL-8 antibody 1 vial 2 vials 3 vials IL-8 Capture antibody 1 x 100µl 2 x 100µl 3 x 100µl IL-8 Standard Yellow 5 vials 10 vials 15 vials Ready-to-use TMB 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
研究领域
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功能
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
细胞定位
Secreted. - Information by UniProt
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别名
- (Ala-IL-8)77
- (Ser-IL-8)72
- 9E3
see all -
数据库链接
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48481于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab48481 被引用在 3 文献中.
- Zhang X et al. Cancer-associated fibroblast-induced lncRNA UPK1A-AS1 confers platinum resistance in pancreatic cancer via efficient double-strand break repair. Oncogene 41:2372-2389 (2022). PubMed: 35264742
- Mollarasouli F et al. Ultrasensitive determination of receptor tyrosine kinase with a label-free electrochemical immunosensor using graphene quantum dots-modified screen-printed electrodes. Anal Chim Acta 1011:28-34 (2018). PubMed: 29475482
- Liang CL et al. Protection Effect of Zhen-Wu-Tang on Adriamycin-Induced Nephrotic Syndrome via Inhibiting Oxidative Lesions and Inflammation Damage. Evid Based Complement Alternat Med 2014:131604 (2014). ELISA . PubMed: 24812565