人IL-8 ELISA试剂盒(ab214030)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.8 pg/ml
- Range: 3.91 pg/ml - 250 pg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-8 ELISA试剂盒
参阅全部 IL-8 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5 1.8% 批次间 样品 n Mean SD CV% Overall 3 7.5% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
1.8 pg/ml -
范围
3.91 pg/ml - 250 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 99 93% - 103% Cell culture media 101 98% - 104% Hep Plasma 87 82% - 91% EDTA Plasma 88 84% - 92% Cit plasma 86 83% - 90% Cerebral Spinal Fluid 89 84% - 94% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human
不与反应: Cow -
产品概述
Human IL-8 ELISA Kit (ab214030) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-8 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-8 with 1.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human IL-8 protein in serum, plasma, and cell culture supernatant samples only.
Cell and tissue extract samples have not been tested with this kit.
SPECIES REACTIVITY
This kit recognizes human IL-8 protein.
Other species reactivity was determined by measuring 100% mouse, rat, and bovine serum samples, interpolating the IL-8 protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the IL-8 protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow
CALIBRATION
This immunoassay is calibrated against a highly purified human IL-8. The NIBSC/WHO unclassified purified human IL-8 preparation 89/520 was evaluated in this kit.
The dose response curve of the unclassified standard IL-8 parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human IL-8 kit to approximate NIBSC IU/mL units, use the equation below.
NIBSC (89/250) approximate value (IU/mL) = 0.0042 x SimpleStep Human IL-8 value (pg/mL).
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说明
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Human IL-8 Capture Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Human IL-8 Detector Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 4BI 1 x 6ml 10 x 6ml 1 x 6ml Human IL-8 Lyophilized Recombinant Protein 2 vials 2 x 10 vials 2 vials Plate Seal 1 unit 1 x 10 units 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究领域
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功能
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
细胞定位
Secreted. - Information by UniProt
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别名
- (Ala-IL-8)77
- (Ser-IL-8)72
- 9E3
see all -
数据库链接
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
相关产品
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Alternative Versions
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ELISA kits
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KO cell lines
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab214030于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Other - Human IL-8 ELISA Kit (ab214030)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Interpolated concentrations of human IL-8 in stimulated PBMC supernatant in 96-well vs. 384-well plates.Interpolated concentration of native IL-8 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.25% stimulated PBMC supernatant. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Example of human IL-8 standard curve in Sample Diluent NS in 96-well vs. 384-well plate.Example of human IL-8 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Human IL-8 standard curve comparison data.Standard curve comparison between human IL-8 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA® kit shows a 3-fold increase in sensitivity.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 61.6 pg/mL with a range of 62 – 174.9 pg/mL; two individuals (Donor #3 and Donor #9) measured below the minimal detectable dose.
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Example of human IL-8 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL-8 standard curve in Sample Diluent NS.The IL-8 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-8 in human serum.The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 63.2 pg/mL in serum.
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Interpolated concentrations of native IL-8 in human PBMC cell culture supernatant.The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 1:400 and stimulated 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Measured values were interpolated from the IL-8 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. The mean IL-8 concentration was determined to be 2.2 ng/mL in unstimulated, 119.7ng/mL in stimulated, and undetectable in media.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IL-8 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant IL-8 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
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Sandwich ELISA - Human IL-8 ELISA Kit (ab214030)Human IL-8 concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-8 ELISA kit (ab214030). Wild-type PC-3 cells and CXCL8 knockout PC-3 cells (ab273743) were assessed in duplicate (n=2) and were either treated with 2 µg/ml LPS for 6 hours to induce expression of IL-8 or not treated with LPS. Data are represented as the mean and error bars represent standard deviation.
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Sandwich ELISA - Human IL-8 ELISA Kit (ab214030)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (34)
ab214030 被引用在 34 文献中.
- Zhang S et al. ANKRD22 aggravates sepsis-induced ARDS and promotes pulmonary M1 macrophage polarization. J Transl Autoimmun 8:100228 (2024). PubMed: 38225946
- Hu S et al. Kinkéliba (Combretum micranthum) Leaf Extract Alleviates Skin Inflammation: In Vitro and In Vivo Study. Molecules 28:N/A (2023). PubMed: 36838778
- Ning L et al. Qingre Xingyu recipe exerts inhibiting effects on ulcerative colitis development by inhibiting TNFα/NLRP3/Caspase-1/IL-1β pathway and macrophage M1 polarization. Cell Death Discov 9:84 (2023). PubMed: 36890151
- Gao L et al. Myeloid-derived growth factor regulates high glucose-mediated apoptosis of gingival fibroblasts and induce AKT pathway activation and nuclear factor κB pathway inhibition. J Dent Sci 18:636-644 (2023). PubMed: 37021271
- Lee S et al. Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells. Front Nutr 10:1162934 (2023). PubMed: 37125026