人IL-6 ELISA试剂盒(ab178013)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.6 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-6 ELISA试剂盒
参阅全部 IL-6 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Supernatant 5 2.1% 批次间 样品 n Mean SD CV% Supernatant 3 2.4% -
样品类型
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
1.6 pg/ml -
范围
7.8 pg/ml - 500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 82 77% - 84% Cell culture media 100 97% - 103% EDTA Plasma 82 80% - 85% Cit plasma 84 81% - 85% Cerebral Spinal Fluid 90 87% - 93% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human IL-6 ELISA kit (ab178013) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 protein in human serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IL-6 with 1.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant Human IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
CROSS REACTIVITY Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and tested for interference. No interference with was observed. 18.Species Reactivity This kit recognizes Human IL-6 protein. Recombinant mouse IL-6 was prepared at 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed. Please contact our Technical Support team for more information
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说明
Interleukin 6 (IL6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL6. IL6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Human IL-6 Detector Antibody 1 x 600µl 10 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 5BI 1 x 6ml 10 x 6ml 1 x 6ml Human IL-6 Capture Antibody (Lyophilized) 1 vial 1 x 10 vials 1 vial Human IL-6 Lyophilized Recombinant Protein (ab9627) 1 x 2 vials 10 x 2 vials 1 x 2 vials Plate Seal 1 unit 10 units 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究领域
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功能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
疾病相关
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
序列相似性
Belongs to the IL-6 superfamily. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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别名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
数据库链接
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
相关产品
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Alternative Versions
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ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178013于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of Human IL-6 standard curve in 96-well and 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentration of native IL-6 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2% Human Stimulated PBMC supernatant treated with 1.5% PHA-M for 46 hrs. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Standard curve comparison between the previous Human IL6 SimpleStep ELISA® kit and the re-developed Human IL6 SimpleStep ELISA® kit. The re-developed kit shows increased sensitivity.
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Background-subtracted data values (mean +/- SD) are graphed.
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Raw data values are shown in the table.
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The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC Cell culture supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL6 concentration was determined to be 60,443 pg/mL in stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.
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Recombinant IL-6 was spiked into biological samples and diluted in a 2-fold dilution series in Sample Diluent NS. NL= Non linear 50% pooled serum and plasma (EDTA, Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
50% serum from ten individual healthy human male and female donors was measured in duplicate. All values were below the detectable range of the assay.
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Native IL-6 was measured in biological samples in a 2- fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Human IL-6 concentration was interpolated from the IL-6 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-6 ELISA kit (ab178013). Wild-type and IL-6 knockout A549 cells (ab273751) were assessed in duplicate (n=2). Cells were either treated with 20 ng/mL active recombinant human IL-1 beta protein (ab259387) for 24 h to induce expression of IL-6 or not treated with IL-1 beta. Data are represented as the mean and error bars represent standard deviation.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (208)
ab178013 被引用在 208 文献中.
- Fan H et al. TopClosure® tension‑relief system improves clinical outcomes of patients with breast cancer undergoing mastectomy. Exp Ther Med 25:70 (2023). PubMed: 36605529
- Wang X et al. Identification of biomarkers and candidate small-molecule drugs in lipopolysaccharide (LPS)-induced acute lung injury by bioinformatics analysis. Allergol Immunopathol (Madr) 51:44-53 (2023). PubMed: 36617821
- Mao Y et al. Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway. Int J Biol Sci 19:593-609 (2023). PubMed: 36632449
- Bahgat DM et al. Essential Oil of Cestrum diurnum L.: GC/MS Analysis, in Vitro and in Silico Anti-HCoV-229E Effects and Inhibitory Activity against LPS-Induced Inflammation. Chem Biodivers 20:e202201045 (2023). PubMed: 36811152
- Yang G & Qiu Y Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial. Medicine (Baltimore) 102:e32384 (2023). PubMed: 36820569