人IL-5 Receptor alpha ELISA试剂盒
Human IL-5 Receptor alpha ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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Human IL-5 Receptor alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-5 Receptor alpha with a sensitivity of 20.55 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
CD125, IL5R, IL5RA, Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125
- sELISA
Supplier Data
Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)
Interpolated concentrations of native IL-5 Receptor alpha in human serum and plasma samples.
The concentrations of IL-5 Receptor alpha were measured in duplicates, interpolated from the IL-5 Receptor alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 Receptor alpha concentration was determined to be 3,175.24 pg/mL in serum, 2,926.77 pg/mL in plasma (citrate), 3,031.12 pg/mL in plasma (heparin), and 2,817.08 pg/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 Receptor alpha concentration was determined to be 2,393.78 pg/mL with a range of 1,175.72 – 4,564.80 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)
Example of human IL-5 Receptor alpha standard curve in Sample Diluent NS.
The IL-5 Receptor alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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反应性数据
产品详情
Human IL-5 Receptor alpha ELISA kit (ab283549) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-5 Receptor alpha protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-5 Receptor alpha with 20.55 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human IL-5 Receptor alpha is a heterodimeric cytokine receptor. Binding of IL-5 activates the beta subunit. It is expressed on eosinophils and basophils and is a target of the drug benralizumab, which leads to almost complete depletion of eosinophils. Benralizumab treatment has been demonstrated in clinical trials to reduce asthma reactions.
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性能和储存信息
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推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IL-5RA plays an important role in the growth differentiation and activation of eosinophils. The protein forms part of the IL-5 receptor complex which binds to the cytokine IL-5 initiating a signaling cascade that results in these processes. Through its interaction with IL-5 IL-5RA influences the survival and function of eosinophils cells critical for responses against parasitic infections and in allergic reactions.
Pathways
IL-5RA is fundamental in the JAK-STAT signaling pathway. This pathway is significant as it facilitates the transduction of extracellular cytokine signals into cellular responses impacting the growth and activity of eosinophils. The IL-5 receptor complex is associated with other cytokine receptors through this pathway involving proteins such as JAK2 and STAT5 which play essential roles in the activation of downstream signaling events and cellular responses.
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