人IL-4 Receptor ELISA试剂盒(ab46022)
Key features and details
- Sensitivity: 11.5 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-4 Receptor ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample A 8 563.7pg/ml 17.8 3.1% Sample B 8 140.6pg/ml 9.6 6.8% 批次间 样品 n Mean SD CV% Sample A 8 530.5pg/ml 35.1 6.6% Sample B 8 106.5pg/ml 7.58 7.1% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 11.5 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human IL-4 Receptor (IL-4R) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-4 receptor molecule (CD124) in Human serum, plasma, buffered solutions or cell culture medium.
A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4R present in the samples.
This kit will recognize both endogenous and recombinant Human IL-4R.
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说明
The standard is affinity purified.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-IL-4R Red 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml IL-4R Microplate (12 x 8 well strips) 1 unit 2 units IL-4R Standard (Lyophilized) Orange 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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相关性
IL-4R binds interleukin 4 and interleukin 13 to regulate IgE production. It also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the IL-4R can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene. -
细胞定位
Cell membrane; single-pass type I membrane protein. Isoform 2: Secreted protein -
别名
- IL 4R alpha
- IL4R
- IL4RA
see all -
数据库链接
- Entrez Gene: 3566 Human
- SwissProt: P24394 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46022于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab46022
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab46022 被引用在 2 文献中.
- Del Nogal Avila M et al. Cytokine storm-based mechanisms for extrapulmonary manifestations of SARS-CoV-2 infection. JCI Insight 8:N/A (2023). PubMed: 37040185
- Xu G et al. Endoscopic removal of nucleus pulposus of intervertebral disc on lumbar intervertebral disc protrusion and the influence on inflammatory factors and immune function. Exp Ther Med 19:301-307 (2020). PubMed: 31853303