人IL-4 ELISA试剂盒High Sensitivity (ab46063)
Key features and details
- Sensitivity: 0.31 pg/ml
- Range: 0.31 pg/ml - 10 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-4 ELISA试剂盒High Sensitivity
参阅全部 IL-4 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 8 7.43pg/ml 0.9 12.3% B 8 6.2pg/ml 0.7 10.8% C 8 8.77pg/ml 0.7 7.6% D 8 7.36pg/ml 0.4 6% 批次间 样品 n Mean SD CV% A 9 7.1pg/ml 0.83 11.8% B 9 6.47pg/ml 0.72 11.1% C 9 8.76pg/ml 1.37 15.7% D 9 7.44pg/ml 1.2 16.1% E 7 2pg/ml 0.874 17% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.31 pg/ml -
范围
0.31 pg/ml - 10 pg/ml -
回收率
101 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 95 97% - 127% Serum 69 61% - 75% -
检测时间
3h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-4 Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in Human serum, plasma, buffered solution and cell culture media.
A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-4.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 200X Wash Buffer White 1 x 10ml Amplification Diluent Brown & blue spot 1 x 15ml Amplifier Yellow 1 x 200µl Biotinylated anti IL-4 1 x 400µl Biotinylated Antibody Diluent Red 1 x 7.5ml Chromogen TMB Substrate Solution 1 x 11ml HRP Diluent Red 1 x 23ml IL-4 Microplate (12 x 8 well strips) 1 unit IL-4 Standard (Lyophilized) 2 vials Stop Reagent Black 1 x 11ml Streptavidin-HRP 2 x 5µl -
研究领域
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相关性
IL-4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL-4. IL-4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL-4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL-4 encoding distinct isoforms have been reported. -
细胞定位
Secreted -
别名
- B cell growth factor 1
- B cell stimulatory factor 1
- BCGF 1
see all -
数据库链接
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46063于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Standard curve of human IL-4, with background signal subtracted (duplicates; +/- SD).
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IL-4 measured in cell culture supernatants from control (PBMC) or treated (PBMC+PHA; 2% PHA-M; LifeTechnologies) cells, grown for 48 hours (duplicates +/- SD). Samples tested neat - 1/10, control supernatant gave signals below level of detection.
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Representative Standard Curve using ab46063
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab46063 被引用在 2 文献中.
- Sun X et al. DNA methylation is involved in the pathogenesis of osteoarthritis by regulating CtBP expression and CtBP-mediated signaling. Int J Biol Sci 16:994-1009 (2020). PubMed: 32140068
- Yang C et al. Inflammation and DNA Methylation-Dependent Down-Regulation of miR-34b-5p Mediates c-MYC Expression and CRL4DCAF4 E3 Ligase Activity in Colitis-Associated Cancer. Am J Pathol 190:674-688 (2020). PubMed: 31972160