JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB215089

人IL-4 ELISA试剂盒

Human IL-4 ELISA Kit

5

(1 Review)

|

(25 Publications)

Human IL-4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-4 with a sensitivity of 1.08 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 20 citations

查看别名

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4

7 Images
ELISA - Human IL-4 ELISA Kit (AB215089)
  • ELISA

Supplier Data

ELISA - Human IL-4 ELISA Kit (AB215089)
Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)

Comparison of IL-4 in unstimulated and PHA-M stimulated human PBMC cell supernatants.

Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of Il-4 were measured in neat supernatant samples in duplicates and interpolated from the IL-4 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 174.6 pg/mL in PHA-M stimulated PBMC cell supernatant, 1.4 pg/mL in unstimulated supernatants and undetectable in media (not shown).

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)

Interpolated concentrations of native IL-4 in PHA-M stimulated human PBMC cell culture supernatant samples.

The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated human PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 174.6 pg/mL in stimulated human PBMC cell culture supernatant.

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)

Example of human IL-4 standard curve in Sample Diluent 75BS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)

Interpolated concentrations of spike recombinant IL-4 in serum and plasma samples.

The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma citrate 100%, plasma EDTA 100%, plasma heparin 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)
  • sELISA

Unknown

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)

Human IL-4 standard curve comparison data.

Standard curve comparison between human IL-4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 8-fold increase in sensitivity.

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4 ELISA Kit (AB215089)

Example of human IL-4 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 1.08 pg/mL

范围

6.25 - 400 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human IL-4 ELISA Kit ab215089 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-4 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, plasma, serum. This SimpleStep sensitivity is 1.08 pg/mL.

How the assay works

Human IL-4 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-4 ELISA Kit ab215089 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-4 antibody [EPR19760-240] - BSA and Azide free (Capture) ab242991
- Anti-IL-4 antibody [EPR19760-17] - BSA and Azide free (Detector) ab242991

IL-4 is a 129 amino acids long secreted protein. IL-4 is a cytokine produced mostly by activated T lymphocytes, mast cells and basophils. It has variety of immune response modulating functions by acting on number of cell types. IL-4 participates in several B-cell activation processes. It is a co-stimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 positively regulates IL31RA expression in macrophages.

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
style
left-column

回收率

[ { "sample": "Serum", "range": "91 - 102 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "103 - 114 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "88 - 98 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "94 - 104 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "112 - 120 %", "average": "= 117" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab215089-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB215089-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215089-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215089-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB215089-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB215089-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BS", "number":"AB215089-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB215089-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-4 Lyophilized Recombinant Protein", "number":"AB215089-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB215089-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-4 Detector Antibody", "number":"AB215089-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-4 Capture Antibody", "number":"AB215089-CMP03", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column
style
left-column

产品实验方案

style
left-column

靶点信息

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
See full target information IL4
style
left-column

文献 (25)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:141 PubMed40102920

2025

Increased melanin induces aberrant keratinocyte - melanocyte - basal - fibroblast cell communication and fibrogenesis by inducing iron overload and ferroptosis resistance in keloids.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangguang Shi,Xueyi Xia,Yang Xiao,Ying Zhang,Yiyi Gong,Yahui Chen,Chenyi Shi,Wei Wang,Jianlan Liu,Jia Huang,Mengguo Liu,Zhuoya Xu,Yanyun Ma,Mengkun Shi,Jiucun Wang,Wenyu Wu

Frontiers in medicine 12:1524198 PubMed40046929

2025

The impact of allergic contact dermatitis on the inflammatory response and repair in wound healing process.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Jiabao Xu,Songyan Qu,Hui Peng

Nutrients 17: PubMed39940325

2025

Immature Sword Bean () Pod Alleviates Allergic Rhinitis (A Double-Blind Trial) Through PI3K/Akt/mTOR Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Jeong Hwang,Hyeock Yoon,Joo-Hyung Cho,Seong Lee,Kyung-A Hwang,Young Jun Kim

American journal of cancer research 14:4803-4816 PubMed39553202

2024

Predicting immunotherapy-related adverse events in late-stage non-small cell lung cancer with KARS G12C mutation treated with PD-1 inhibitors through combined assessment of LCP1 and ADPGK expression levels.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Chen,Cong Xu,Weihao Ren,Lei Yu,Tian Tang

Molecular neurodegeneration 19:80 PubMed39456006

2024

Repetitive transcranial magnetic stimulation alleviates motor impairment in Parkinson's disease: association with peripheral inflammatory regulatory T-cells and SYT6.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Xie,Bibiao Shen,Yuqi Luo,Hang Zhou,Zhenchao Xie,Shuzhen Zhu,Xiaobo Wei,Zihan Chang,Zhaohua Zhu,Changhai Ding,Kunlin Jin,Chengwu Yang,Lucia Batzu,K Ray Chaudhuri,Ling-Ling Chan,Eng-King Tan,Qing Wang

World journal of gastrointestinal oncology 16:3118-3157 PubMed39072171

2024

Kombo knife combined with sorafenib in liver cancer treatment: Efficacy and safety under immune function influence.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Cao,Pei-Pei Li,Bing-Li Qiao,Quan-Wang Li

American journal of translational research 16:2670-2682 PubMed39006280

2024

Screening and analysis for potential clinical diagnostic and prognostic markers in allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yejun Liu,Yonggang Kong,Xuhong Zhou

The British journal of nutrition 132:738-749 PubMed38751303

2024

A 12-week double-blind randomised controlled trial investigating the effect of dietary supplementation with 125 g/d vitamin D in adults with asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Watkins,Tanja Harrison,Sohail Mushtaq

Experimental and therapeutic medicine 26:359 PubMed37324510

2023

CX3CL1/CX3CR1 axis alleviates inflammation and apoptosis in human nucleus pulpous cells via M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Wen Gao,Han-Lin Hu,Ming-Hua Xie,Cai-Xia Tang,Jun Ou,Zheng-Hao Lu

American journal of translational research 15:1360-1366 PubMed36915768

2023

Therapeutic effect of modified Xiaoqinglong Decoction on cough-variant asthma and immunological functioning in children.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Ying Han
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB185986

Human IL-10 ELISA Kit (Interleukin-10)

KO Validated|Recombinant|SimpleStep

ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

  • 0
  • 0
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
Assay Kits

AB174443

Human IFN gamma ELISA Kit

SimpleStep

ELISA - Human IFN gamma ELISA Kit (AB174443)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com