人IL-31 ELISA试剂盒(ab273163)
Key features and details
- Sensitivity: 18.3 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Other biological fluids, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-31 ELISA试剂盒
参阅全部 IL-31 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Various 2.5% 批次间 样品 n Mean SD CV% Various 5.2% -
样品类型
Cell culture supernatant, Serum, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 18.3 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 98 82% - 120% -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human IL-31 ELISA kit (ab273163) for the in vitro qualitative and quantitative determination of IL-31 in supernatants, buffered solutions or serum and plasma samples and other body fluids. This assay will recognise both natural and recombinant human IL-31.
A capture Antibody highly specific for IL-31 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of IL-31 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.
During the next incubation period the binding of the biotinylated anti-IL-31 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IL-31 present in the samples and standards.
The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IL-31 in any sample tested.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate (12 x 8 well strips)
性能
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存放说明
Please refer to protocols. -
组件 标识符 1 x 96 tests Biotinylated Antibody diluent 1 x 7ml Biotinylated Antibody IL-31 1 x 400µl HRP Diluent Red 1 x 12ml IL-31 Coated Microwell strips 1 unit IL-31 Standard 2 vials Plastic Plate Cover 2 units Standard diluent Buffer 1 x 15ml Stop Reagent 1 x 11ml Streptavidin-HRP 2 x 5µl TMB Substrate 1 x 11ml Wash Buffer 1 x 10ml -
研究领域
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功能
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. -
组织特异性
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. -
细胞定位
Secreted. - Information by UniProt
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别名
- IL 31
- IL-31
- IL31
see all -
数据库链接
- Entrez Gene: 386653 Human
- Omim: 609509 Human
- SwissProt: Q6EBC2 Human
- Unigene: 569071 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273163于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of Human IL-31 ELISA kit standard curve.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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