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AB235637

人IL-31 ELISA试剂盒

Human IL-31 ELISA Kit

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Human IL-31 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-31 with a sensitivity of 29.83 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Interleukin-31, IL-31, IL31

3 Images
Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

Interpolated concentrations of spike IL-31 in human serum, plasma (EDTA), and cell culture media (RPMI containing 10% FBS) samples.

The concentrations of IL-31 were measured in duplicates, interpolated from the IL-31 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (EDTA) 25%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

Example of human IL-31 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

Example of human IL-31 standard curve in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 29.83 pg/mL

范围

117.2 - 7500 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human IL-31 ELISA Kit (ab235637) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-31 protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-31 with 1.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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回收率

[ { "sample": "Serum", "range": "98 - 102 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "92 - 98 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "103 - 109 %", "average": "= 105" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR (PubMed : 15184896). May function in skin immunity (PubMed : 15184896). Enhances myeloid progenitor cell survival in vitro (By similarity). Induces RETNLA and serum amyloid A protein expression in macrophages (By similarity).
See full target information IL31
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websiteProtocolBooklet
en
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