JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB100567

人IL-28A ELISA试剂盒(Interleukin-28A)

Human IL-28A ELISA Kit (Interleukin-28A)

Be the first to review this product! Submit a review

|

(0 Publication)

Human IL-28A ELISA Kit (Interleukin-28A) is a Sandwich ELISA for the measurement of Human IL-28A (Interleukin-28A) in Human in Biofluids, Cell Culture Media samples.

查看别名

IL28A, ZCYTO20, IFNL2, Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A

1 Images
Sandwich ELISA - Human IL-28A ELISA Kit (Interleukin-28A) (AB100567)
  • sELISA

Unknown

Sandwich ELISA - Human IL-28A ELISA Kit (Interleukin-28A) (AB100567)

Representative standard curve using ab100567

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

< 15 pg/mL

范围

13.72 - 10000 pg/mL

检测平台

Microplate

style
left-column

搭配使用产品

Assay Kits

AB222510

Human VEGF ELISA Kit

SimpleStep

ELISA - Human VEGF ELISA Kit (AB222510)

  • 5
  • 1
Assay Kits

AB215088

Human IL-3 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-3 ELISA Kit (AB215088)

  • 0
  • 0
Assay Kits

AB100568

Human IL-29 ELISA Kit

Sandwich ELISA - Human IL-29 ELISA Kit (AB100568)

  • 0
  • 0
Assay Kits

AB282870

Mouse IL-21 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Abcam's IL-28A (Interleukin 28A) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28A in plasma and cell culture supernatants.

This assay employs an antibody specific for Human IL-28A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-28A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human IL-28A in normal serum with ab100567, only in serum spiked with Human IL-28A.

Optimization may be required with urine samples.

style
left-column

回收率

[ { "sample": "Cell culture supernatant", "range": "95 - 114 %", "average": "= 105.2" }, { "sample": "Plasma", "range": "84 - 120 %", "average": "= 110.4" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100567-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100567-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100567-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100567-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-28A Microplate (12 x 8 wells)", "number":"AB100567-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100567-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100567-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-28A", "number":"AB100567-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-28A Standard (lyophilized)", "number":"AB100567-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100567-CMP04", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
style
left-column
style
left-column

产品实验方案

style
left-column

靶点信息

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information IFNL2
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com