人IL-23 ELISA试剂盒(ab64708)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 156.25 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-23 ELISA试剂盒
参阅全部 IL-23 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 6 4637pg/ml 72 1.6% B 6 3908pg/ml 36 1% C 6 3016pg/ml 72 2.4% 批次间 样品 n Mean SD CV% A 9 4439pg/ml 276 6.2% B 9 3807pg/ml 353 9.2% C 9 2570pg/ml 388 15% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
156.25 pg/ml - 5000 pg/ml -
回收率
89 %
特定样本回收率 样品类型 平均% 范围 Serum 118 103% - 134% -
检测时间
2h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-23 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-23 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
A monoclonal antibody specific for IL-23 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-23 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-23 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-23 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-23.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-IL-23 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml IL-23 Microplate(12 x 8 well strips) 1 unit 2 units IL-23 Standard (Lyophilized) Yellow 2 vials 4 vials Plastic Plate Covers 2 units 4 units Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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功能
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
组织特异性
Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). -
序列相似性
Belongs to the IL-6 superfamily. -
发展阶段
Expressed by newborns dendritic cells. -
细胞定位
Secreted. Secreted upon association with IL12B. - Information by UniProt
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别名
- IL 23
- IL 23 A
- IL 23 subunit alpha
see all -
数据库链接
- Entrez Gene: 51561 Human
- Entrez Gene: 3593 Human
- Omim: 605580 Human
- Omim: 161561 Human
- SwissProt: Q9NPF7 Human
- SwissProt: P29460 Human
- Unigene: 382212 Human
- Unigene: 98309 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64708于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab64708
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab64708 被引用在 4 文献中.
- Yang G & Qiu Y Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial. Medicine (Baltimore) 102:e32384 (2023). PubMed: 36820569
- Domfeh SA et al. Cryptolepine inhibits hepatocellular carcinoma growth through inhibiting interleukin-6/STAT3 signalling. BMC Complement Med Ther 21:161 (2021). PubMed: 34078370
- Yu X et al. Intensive Cytokine induction in Pandemic H1N1 Influenza Virus Infection Accompanied by Robust Production of IL-10 and IL-6. PLoS One 6:e28680 (2011). ELISA . PubMed: 22174866
- Abe T et al. Combination use of immune complexes and a Ca2(+) channel blocker azelnidipine enhances interleukin-12 p40 secretion without T helper type 17 cytokine secretion in human monocyte-derived dendritic cells. Clin Exp Immunol 156:405-12 (2009). ELISA . PubMed: 19438591