人IL-2 ELISA试剂盒(ab46033)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-2 ELISA试剂盒
参阅全部 IL-2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample 6 3.4% 批次间 样品 n Mean SD CV% Sample 6 9.4% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
回收率
94 %
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检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in supernatants, buffered soloutions, serum and plasma samples.
A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-2.
Get results in 90 minutes with Human IL-2 ELISA Kit (ab174444) from our SimpleStep ELISA® range.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated anti IL-2 Red 1 x 400µl 2 x 400µl Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml IL-2 Microplate (12 x 8 well strips) 1 unit 2 units IL-2 Standard (Lyophilized) Yellow 2 vials 4 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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功能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
疾病相关
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
序列相似性
Belongs to the IL-2 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Aldesleukin
- IL 2
- IL-2
see all -
数据库链接
- Entrez Gene: 3558 Human
- Omim: 147680 Human
- SwissProt: P60568 Human
- Unigene: 89679 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab46033 被引用在 1 文献中.
- Tumas S et al. Engineered E. coli Nissle 1917 for delivery of bioactive IL-2 for cancer immunotherapy. Sci Rep 13:12506 (2023). PubMed: 37532747