人IL-18 R beta ELISA试剂盒(ab113325)
Key features and details
- Sensitivity: 12 pg/ml
- Range: 12.3 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-18 R beta ELISA试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 12 pg/ml -
范围
12.3 pg/ml - 3000 pg/ml -
回收率
77 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 75.78 68% - 85% Serum 80.9 71% - 89% Plasma 74.98 67% - 83% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-18 R beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 R beta in serum, plasma, cell culture supernatants. (Human IL-18 R beta concentration is pretty low in normal serum/plasma, it may not be detected in this assay)
This assay employs an antibody specific for Human IL-18 R beta coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Human IL-18 R beta 2 vials IL-18 R beta Microplate 1 unit Recombinant Human IL-18 R beta Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex (By similarity). Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. -
组织特异性
Strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells. -
序列相似性
Belongs to the interleukin-1 receptor family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
细胞定位
Membrane. - Information by UniProt
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别名
- IL18RAP
- Accessory protein like
- Accessory protein-like
see all -
数据库链接
- Entrez Gene: 8807 Human
- Omim: 604509 Human
- SwissProt: O95256 Human
- Unigene: 158315 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113325于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Representative Standard Curve
Representative Standard Curve using ab113325
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab113325 尚未被引用在任何文献中。