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AB243670

人IL-17B ELISA试剂盒

Human IL-17B ELISA Kit

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Human IL-17B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-17B with a sensitivity of 3.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

IL20, NIRF, ZCYTO7, UNQ516/PRO1031, IL17B, Interleukin-17B, IL-17B, Cytokine Zcyto7, Interleukin-20, Neuronal interleukin-17-related factor, IL-20

2 Images
Sandwich ELISA - Human IL-17B ELISA Kit (AB243670)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-17B ELISA Kit (AB243670)

Interpolated concentrations of spike IL-17B in human serum, plasma and cell culture media (RPMI 1640 containing 10% FBS) samples.

The concentrations of IL-17B were measured in duplicates, interpolated from the IL-17B standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and cell culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-17B ELISA Kit (AB243670)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-17B ELISA Kit (AB243670)

Example of human IL-17B standard curve in Sample Diluent NS.

The IL-17B standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 3.6 pg/mL

范围

18.8 - 1200 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human IL-17B ELISA Kit (ab243670) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17B protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17B with 3.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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回收率

[ { "sample": "Serum", "range": "84 - 97 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "74 - 88 %", "average": "= 82" }, { "sample": "Citrate plasma", "range": "78 - 92 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "87 - 97 %", "average": "= 92" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243670-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB243670-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB243670-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243670-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243670-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243670-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243670-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243670-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-17B Lyophilized Recombinant Protein", "number":"AB243670-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17B Capture Antibody", "number":"AB243670-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17B Detector Antibody", "number":"AB243670-CMP02", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.
See full target information IL17B
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websiteProtocolBooklet
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