人IL-17AF ELISA试剂盒(ab273162)
Key features and details
- Sensitivity: 6.6 pg/ml
- Range: 15.6 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-17AF ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 9 230.3pg/ml 8.1 3.5% 2 9 313.4pg/ml 19.2 6.1% 3 9 435.5pg/ml 32.4 7.5% 批次间 样品 n Mean SD CV% 1 9 230.1pg/ml 9.7 4.2% 2 9 313.2pg/ml 20.1 6.4% 3 9 435.3pg/ml 31.3 7.2% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
6.6 pg/ml -
范围
15.6 pg/ml - 500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 102 77% - 117% -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human IL-17AF ELISA kit (ab273162) is for the in vitro qualitative and quantitative determination of IL-17AF in supernatants, buffered solutions or serum and plasma samples and other body fluids. This assay will recognise both natural and recombinant human IL-17AF.
A highly specific capture antibody has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of IL-17AF in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.
During the next incubation period the binding of the biotinylated secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IL-17AF present in the samples and standards.
The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IL-17AF in any sample tested.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests Biotinylated Antibody diluent 1 x 7ml Biotinylated Antibody IL-17AF 1 x 400µl HRP Diluent Red 1 x 12ml IL-17AF Coated Microwell strips 1 unit IL-17AF Standard 2 vials Plastic Plate Cover 2 units Standard diluent Buffer 1 x 15ml Stop Reagent 1 x 11ml Streptavidin-HRP 2 x 5µl TMB Substrate 1 x 11ml Wash Buffer 1 x 10ml -
相关性
Interleukin-17AF (IL-17AF) is a member of the IL-17 family of proteins produced by a subset of T cells, called Th17, following stimulation with IL-23. Since IL-17AF is thought to signal through the IL-17R receptor, its biological function is similar to that of IL-17A in that it induces the production of a variety of chemokines, in addition to airway neutrophilia. In regard to these functions, IL-17AF has less activity than the IL-17A homodimer but, greater activity than the IL-17F homodimer. Human and rat IL-17AF both show activity on mouse cells. -
数据库链接
- SwissProt: Q16552 Human
- SwissProt: Q96PD4 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273162于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of Human IL-17AF ELISA kit standard curve.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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