人IL-17 ELISA试剂盒(ab119535)
Key features and details
- Sensitivity: 0.5 pg/ml
- Range: 1.6 pg/ml - 100 pg/ml
- Sample type: Cell culture extracts, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-17 ELISA试剂盒
参阅全部 IL-17A 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 7.1% 批次间 样品 n Mean SD CV% Overall 9.1% -
样品类型
Serum, Plasma, Cell culture extracts -
检测类型
Sandwich (quantitative) -
灵敏度
0.5 pg/ml -
范围
1.6 pg/ml - 100 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 113.6 % - % Serum 107 % - % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-17 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-17 concentrations in Cell culture supernatant, serum and plasma (citrate, heparin).
Human IL-17 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human IL-17 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human IL-17 captured on the plate.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-Human IL-17 monoclonal antibody 1 x 70µl Control high (80 - 220 pg/ml) 1 vial Control low (5 - 20 pg/ml) 1 vial Human IL-17 Standard Lyophilized (200 pg/mL on reconstitution) 2 vials Microplate coated with monoclonal antibody to Human Il-17 (12 x 8 wells) 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究领域
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功能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
组织特异性
Restricted to activated memory T-cells. -
序列相似性
Belongs to the IL-17 family. -
翻译后修饰
Found both in glycosylated and nonglycosylated forms. -
细胞定位
Secreted. - Information by UniProt
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别名
- CTLA 8
- CTLA-8
- CTLA8
see all -
数据库链接
- Entrez Gene: 3605 Human
- Omim: 603149 Human
- SwissProt: Q16552 Human
- Unigene: 41724 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119535于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab119535.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab119535 被引用在 11 文献中.
- Yang G & Qiu Y Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial. Medicine (Baltimore) 102:e32384 (2023). PubMed: 36820569
- Shill MC et al. Screening of plasma IL-6 and IL-17 in Bangladeshi lung cancer patients. Heliyon 9:e20471 (2023). PubMed: 37810816
- Al-Bishri WM Glucose transporter 1 deficiency, AMP-activated protein kinase activation and immune dysregulation in autism spectrum disorder: Novel biomarker sources for clinical diagnosis. Saudi J Biol Sci 30:103849 (2023). PubMed: 38020228
- Kinoshita N et al. Serum IL-13 Predicts Response to Golimumab in Bio-Naïve Ulcerative Colitis. J Clin Med 11:N/A (2022). PubMed: 36078882
- He S et al. Hsa_circ_0010957 level is increased and sponges microRNA-125b in CD4+ T cells of patients with systemic lupus erythematosus. Mol Med Rep 23:N/A (2021). PubMed: 33880592