AB100550

人IL-10RB ELISA试剂盒

Name: 人IL-10RB ELISA试剂盒 (ab100550)| Abcam中文官网
Brand: Abcam Limited
SKU: AB100550
Price: 7672 CNY
Availability: InStock

Human IL-10RB ELISA Kit

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Human IL-10RB ELISA Kit is a Sandwich ELISA for the measurement of Human IL-10RB in Human in Biofluids, Cell Culture Media samples.

查看别名

CDw210b, CRFB4, D21S58, D21S66, IL10RB, Interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, Cytokine receptor class-II member 4, Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, CRF2-4, IL-10R subunit 2, IL-10R2

2 Images

Sandwich ELISA - Human IL-10RB ELISA Kit (AB100550)

sELISA

Unknown

Sandwich ELISA - Human IL-10RB ELISA Kit (AB100550)

Representative standard curve using ab100550

sELISA

Unknown

Sandwich ELISA - Human IL-10RB ELISA Kit (AB100550)

Representative standard curve using ab100550

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

灵敏度

< 5 pg/mL

范围

4.12 - 3000 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's IL-10RB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IL-10RB in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human IL-10RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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回收率

[ { "sample": "Cell culture supernatant", "range": "120 - 140 %", "average": "= 129.1" }, { "sample": "Serum", "range": "110 - 129 %", "average": "= 121.9" }, { "sample": "Plasma", "range": "92 - 112 %", "average": "= 104.5" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100550-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100550-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-10RB", "number":"AB100550-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100550-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100550-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100550-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100550-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100550-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-10RB Standard (lyophilized)", "number":"AB100550-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10RB Microplate (12 x 8 wells)", "number":"AB100550-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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产品实验方案

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靶点信息

Shared cell surface receptor required for the activation of five class 2 cytokines : IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.

See full target information IL10RB

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websiteProtocolBooklet

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Abcam Product Promise

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For licensing inquiries, please contact partnerships@abcam.com

人IL-10RB ELISA试剂盒

Human IL-10RB ELISA Kit

Sandwich ELISA - Human IL-10RB ELISA Kit (AB100550)

Sandwich ELISA - Human IL-10RB ELISA Kit (AB100550)