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AB185986

人IL-10 ELISA试剂盒(Interleukin-10)

Human IL-10 ELISA Kit (Interleukin-10)

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(49 Publications)

Human IL-10 ELISA kit (Interleukin-10) is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human IL-10 with a sensitivity of 1.4 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450 nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Broad sample compatibility – works with serum, plasma, and cell culture supernatants
- Cited in over 30 publications

查看别名

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

9 Images
ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
  • ELISA

Supplier Data

ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

Example of human IL-10 standard curve in Sample Diluent NS + Enhancer.

The IL-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

KO data.

Human IL-10 concentration was interpolated from the IL-10 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-10 ELISA Kit (Interleukin-10) (ab185986). Wild-type and IL-10 knockout THP-1 (ab273763) vehicle control and PMA (100 ng/mL, 56 h) + LPS (1 μg/mL, 24 h) treated cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation."

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

Human IL-10 Standard Curve Comparison.

Standard Curve comparison between human IL-10 current (new) SimpleStep ELISA kit and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

Interpolated concentration of native IL-10 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% stimulated PBMC supernatant (1.5% PHA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

Example of human IL-10 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

Interpolated concentrations of native IL-10 in human PBMC cell culture supernatant samples.

The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 462 pg/ml in stimulated PBMC cell culture supernatant. PBMC cell culture supernatant samples were stimulated for 46 hours with 1.5% PHA. Unstimulated PBMC cell culture supernatant samples were also measured; all values were below the detectable range of the assay.

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

Example of human IL-10 standard curve in Sample Diluent 50BP + Enhancer.

The IL-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

Example of human IL-10 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 1.4 pg/mL

范围

9.4 - 3000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human IL-10 ELISA Kit (Interleukin-10) ab185986 is a rapid single-wash 90-min ELISA kit to measure Human IL-10 in cell culture supernatant, serum, plasma. This SimpleStep ELISA® sensitivity is 1.4 pg/mL.

How the assay works
Human IL-10 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit is designed for the quantification of human IL-10. The standard protein in this kit is mature full length human IL-10. Native signal was detected in cell culture supernatant. Spike protein experiments were used to validate serum and plasma samples. Saliva, milk, CSF, cell extract and tissue extract samples have not bee tested with this kit. 50% pooled serum and plasma samples from healthy donors were measured in duplicate. All values were below the detectable range of this assay. 50% serum from 10 healthy human male donors was measured in duplicate. All values were below the detectable range of this assay.

Human IL-10 ELISA Kit (Interleukin-10) ab185986 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IL-10 ELISA Kit ab185986
Human IL-10 ELISA Kit ab185986 has been used to assess inflammation in different physio-pathological contexts such as celiac disease(1), tuberculosis(2), and atherosclerosis(3).

References:
(1)Soroush Sardari et al. 2023, PMID: 38040898,
(2)Hao Zeng et al. 2023, PMID: 37601387,
(3)Youjiang Qiu et al. 2023, PMID: 36820569.

Save your precious samples
Our 384-well Human IL-10 ELISA Kit (Interleukin-10) only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human IL-10 Antibody Pair - BSA and Azide free (Interleukin-10) ab243976
- Anti-IL-10 antibody [EPR20433-201] - BSA and Azide free (Capture) ab244835
- Anti-IL-10 antibody [EPR20433-290] - BSA and Azide free (Detector) ab244889

Related and recommended products
Human IL-10 ELISA Kit ab185986 is commonly used to measure IL-10 as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA kit ab181421
- Human IL-8 ELISA kit ab214030
- Human IL-6 ELISA kit ab178013

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精确度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "105 - 111 %", "average": "= 108" }, { "sample": "Serum", "range": "108 - 112 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "103 - 118 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "113 - 117 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "105 - 119 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "102 - 111 %", "average": "= 106" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information IL10
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文献 (49)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:463 PubMed40541951

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ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma.

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Lei Ye,Genyi Jiang,Yihan Sun,Bilan Li

American journal of translational research 17:2197-2209 PubMed40226016

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Development of a predictive model for the relationship between serum pan-immunoinflammatory index levels and scar formation in facial burn patients.

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Di Xi,Hailun Yu,Tong Yu

Immunologic research 73:69 PubMed40199768

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HBx/WDR5 enhances IGF-1 transcription in hepatocellular carcinoma cells and promotes recruitment, infiltration, and activity of Treg cells.

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Erli Wang,Shuhua Sun,Hui Li,Yi Jia,Zhe Bai

BMC cardiovascular disorders 25:192 PubMed40098068

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Effects of low-dose radiation produced during radiofrequency ablation guided by 3D mapping on mitochondrial apoptosis in diabetic cardiomyocytes.

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Jia Gao,Zhijun Meng,Binghang Zhang,Nan Zhang,Min Guo,Meng Sun,Rui Wang

Frontiers in medicine 12:1524198 PubMed40046929

2025

The impact of allergic contact dermatitis on the inflammatory response and repair in wound healing process.

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Wei Zhang,Jiabao Xu,Songyan Qu,Hui Peng

American journal of cancer research 15:153-167 PubMed39949943

2025

Risk factors and prediction model for cancer-related cognitive impairment in thyroid cancer patients.

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Ting Ni,Jie Sun,Qin He,Yuning Dai,Xiaobei Wang,Enqiao Yu,Guodong Shen

Journal of bone oncology 50:100655 PubMed39850453

2025

Using β-Elemene to reduce stemness and drug resistance in osteosarcoma: A focus on the AKT/FOXO1 signaling pathway and immune modulation.

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Shaochun Zhang,Zhijie Xing,Jing Ke

Infection and immunity 93:e0027124 PubMed39841046

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MASP1 modulation as a novel therapeutic target in severe pediatric pertussis: insights from a multi-omics approach.

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Lin Xu,Caiying Wang,Yuhuan Liu,Yanlan Zhang,Zhen Li,Lin Pang

American journal of translational research 16:7618-7632 PubMed39822549

2025

Improvement of upper limb function in post-stroke patients with motion feedback training-based combination therapy: a retrospective analysis of muscle activation and recovery dynamics.

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Species

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Shouzhang Wang,Kun Yang,Dongqin Zhu,Rongli Rao

American journal of cancer research 14:5798-5811 PubMed39803642

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Construction of a predictive model for the effectiveness of plastic surgery and repair in patients with facial basal cell carcinoma.

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Lijun Wu,Shuhan Tang,Jingting Jiang,Ke Li
View all publications
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websiteProtocolBooklet
en
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搭配使用产品

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Secondary Antibodies

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