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AB325446

Human IL-10 ELISA Kit, Chemiluminescent

Human IL-10 ELISA Kit, Chemiluminescent

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Human IL-10 ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human IL-10 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :6.2 - 4500 pg/mL with a sensitivity of 1 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- The antibodies used in this kit are knock out validated for optimal specificity

查看别名

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

6 Images
Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)

Standard curve comparison between the Chemiluminescent Human IL-10 SimpleStep ELISA and colorimetric Human IL-10 SimpleStep ELISA (ab185986) in Sample Diluent 50BP + 1X Enhancer. Background -subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)

Interpolated concentrations of native IL-10 in human PBMC cell culture supernatant samples. The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 462 pg/ml in stimulated PBMC cell culture supernatant. PBMC cell culture supernatant samples were stimulated for 46 hours with 1.5% PHA. Unstimulated PBMC cell culture supernatant samples were also measured; all values were below the detectable range of the assay.

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)

Dynamic range comparison between the Chemiluminescent Human IL-10 SimpleStep ELISA and colorimetric Human IL-10 SimpleStep ELISA (ab185986).

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)

Standard curve comparison between the Chemiluminescent Human IL-10 SimpleStep ELISA and colorimetric Human IL-10 SimpleStep ELISA (ab185986) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)
  • sELISA

Lab

Sandwich ELISA - Human IL-10 ELISA Kit, Chemiluminescent (AB325446)

Human IL-10 concentration was interpolated from the IL-10 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-10 ELISA Kit (Interleukin-10) (ab185986). Wild-type and IL-10 knockout THP-1 (ab273763) vehicle control and PMA (100 ng/mL, 56 h) + LPS (1 μg/mL, 24 h) treated cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.

关键信息

检测方法

Luminescent

样品类型

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 1 pg/mL

范围

6.2 - 4500 pg/mL

检测时间

55m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

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产品详情

Human IL-10 ELISA Kit, Chemiluminescent (ab325446) is a rapid single-wash sandwich 55-min ELISA to measure Human IL-10 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant with a sensitivity of 1 pg/ml.

How the assay works

Human IL-10 ELISA Kit, Chemiluminescent (ab325446) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human IL-10 (ab325446) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-10 antibody [EPR20433-201] BSA and Azide free (Capture) ab244835

- Anti-IL-10 antibody [EPR20433-290] BSA and Azide free (Detector) ab244889

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.2" } ]
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回收率

[ { "sample": "Serum", "range": "108 - 112 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "105 - 119 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "103 - 118 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "113 - 117 %", "average": "= 116" }, { "sample": "Cell culture supernatant", "range": "105 - 111 %", "average": "= 108" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325446-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human IL-10 Capture Antibody", "number":"AB325446-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-10 Detector Antibody", "number":"AB325446-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325446-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325446-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB325446-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325446-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325446-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325446-CMP12", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325446-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325446-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent NBP", "number":"AB325446-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-10 Lyophilized Recombinant Protein", "number":"AB325446-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information IL10
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websiteProtocolBooklet
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