人IL-1 beta ELISA试剂盒

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Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

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Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

THP-1 cells were cultured unstimulated or stimulated with 5 µg/mL Lipopolysaccharide (LPS).

Conditioned media was harvested after 48 hours. IL-1beta was measured in 100% unstimulated and LPS stimulated THP-1 supernatant. The concentrations of IL-1beta were measured in duplicate and interpolated from the IL-1beta standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 718 pg/mL in LPS stimulated THP-1 supernatant. There was no detectable signal in unstimulated supernatant.

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Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384).

This data was collected using ab229384, which uses the same recombinant antibody pair and standard protein. Human IL-1 beta concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-1 beta ELISA kit (ab229384). Wild-type and IL-1 beta knockout THP-1 cells (ab273762) were assessed in duplicate (n=2). Cells were either treated with LPS (100 ng/ml, 3 h) then ATP (5 mM, 45 min) to induce expression of IL-1 beta or not treated. Data are represented as the mean and error bars represent standard deviation.

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Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated values are plotted (mean +/- SD, n=2). IL-1beta was measured in 2 donor serum samples (30 pg/mL and 140 pg/mL) and the remaining 8 samples measured less than the lowest point of the IL-1beta standard curve.

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Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

Example of human IL-1beta standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

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Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

Linearity of dilution.

Recombinant IL-1beta was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

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Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.

Conditioned media was harvested after 48 hours. IL-1beta was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-1beta were measured in duplicate, interpolated from the IL-1beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1273 pg/mL in PHA stimulated PBMC supernatant. There was no detectable signal in unstimulated supernatant.

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Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

Example of human IL-1beta standard curve in Sample Diluent NS. The IL-1beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

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Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native IL-1beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

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Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

Interpolated concentrations of native IL-1beta in human PHA stimulated PBMC supernatant and LPS stimulated THP-1 supernatant samples.

The concentrations of IL-1beta were measured in duplicates, interpolated from the IL-1beta standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 50% and 100% THP-1 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1301 pg/mL in PBMC supernatant and 734 pg/mL in THP-1 supernatant.