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AB313974

人IKB beta ELISA试剂盒

Human IKB beta ELISA Kit

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Human IKB beta ELISA Kit is a Sandwich ELISA for the measurement of Human IKB beta in Human in Biofluids, Cell Culture Media samples.

查看别名

IKBB, TRIP9, NFKBIB, NF-kappa-B inhibitor beta, NF-kappa-BIB, I-kappa-B-beta, Thyroid receptor-interacting protein 9, IkB-B, IkB-beta, IkappaBbeta, TR-interacting protein 9, TRIP-9

1 Images
Sandwich ELISA - Human IKB beta ELISA Kit (AB313974)
  • sELISA

Supplier Data

Sandwich ELISA - Human IKB beta ELISA Kit (AB313974)

Example of standard curve using ab313974. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Serum, Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich

灵敏度

= 0.55 ng/mL

范围

0.55 - 500 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Human IKB beta ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IKB beta in serum, plasma (collect plasma using EDTA and Heparin as an anticoagulant. Citrate is not recommended in this assay), and cell culture supernatants. This assay employs an antibody specific for human IKB beta coated on a 96-well plate.

回收率

[ { "sample": "Serum", "range": "107 - 136 %", "average": "= 120.7" }, { "sample": "Plasma", "range": "71 - 82 %", "average": "= 75.12" }, { "sample": "Cell culture media", "range": "126 - 140 %", "average": "= 133.4" } ]

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313974-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313974-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313974-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313974-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"350X HRP-Streptavidin", "number":"AB313974-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human IKB beta Antibody-coated ELISA Plate", "number":"AB313974-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human IKB beta Protein Standard", "number":"AB313974-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313974-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human IKB beta Detection Antibody", "number":"AB313974-CMP09", "productcode":"" } ] } } }

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C

产品实验方案

靶点信息

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
See full target information NFKBIB
websiteProtocolBooklet
en

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