AB320788

Human IgG4 ELISA Kit

Recombinant
SimpleStep
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Human IgG4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IgG4 with a sensitivity of 40.048 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4

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Sandwich ELISA - Human IgG4 ELISA Kit (AB320788)

sELISA

Supplier Data

Sandwich ELISA - Human IgG4 ELISA Kit (AB320788)

Interpolated concentrations of human IgG4 in serum in 96-well vs. 384-well plates. Interpolated concentration of native IgG4 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 100,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human IgG4 ELISA Kit (AB320788)

Interpolated concentrations of human IgG4 in urine, saliva, and milk. Interpolated concentration of native IgG4 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 1 : 20, saliva 1 : 100, and milk 1 : 100. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human IgG4 ELISA Kit (AB320788)

Cross reactivity of related immunoglobulin proteins. 50 ng/mL of natively purified human IgG1, IgG2, IgG3, IgA, IgD, IgE, and IgM were tested individually for cross-reactivity. Percent reactivity was determined by interpolating the protein concentration from the human IgG4 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested.

sELISA

Supplier Data

Sandwich ELISA - Human IgG4 ELISA Kit (AB320788)

Interpolated concentrations of IgG4 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean IgG4 concentration was determined to be 248 µg/mL with a range of 102 – 566 µg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IgG4 ELISA Kit (AB320788)

Interpolated concentrations of human IgG4 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native IgG4 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 100,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human IgG4 ELISA Kit (AB320788)

Example of human IgG4 standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Example of human IgG4 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IgG4 ELISA Kit (AB320788)

Example of human IgG4 standard curve in 1X Wash Buffer PT. Example of human IgG4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk

反应种属

Human

检测类型

Sandwich

Quantitative

灵敏度

= 40.048 pg/mL

范围

140.625 - 9000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human IgG4 ELISA kit (ab320788) is a rapid single-wash sandwich 90-min ELISA to measure Human IgG4 in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk. This SimpleStep sensitivity is 40.048 pg/mL.

How the assay works

Human IgG4 ELISA kit (ab320788) SimpleStep ELISA^® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IgG4 (ab320788) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Human IgG4 ELISA kit only requires 12.5µL sample.

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IgG4 antibody [EPR26764-64] - BSA and Azide free (Capture) ab309230
- Anti-IgG4 antibody [EPR26764-24] - BSA and Azide free (Detector) ab309231

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" } ]

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回收率

[ { "sample": "Serum", "range": "81 - 91 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "103 - 114 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "98 - 106 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "114 - 115 %", "average": "= 114" }, { "sample": "Urine", "range": "87 - 105 %", "average": "= 94" }, { "sample": "Saliva", "range": "99 - 121 %", "average": "= 108" }, { "sample": "Milk", "range": "84 - 104 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "89 - 90 %", "average": "= 90" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

IgG4 also known as IgG4-Fc or IGHG4 is one of the subclasses of Immunoglobulin G (IgG) antibodies. Mechanically it plays a role in the immune response by recognizing and binding to specific antigens. Its molecular weight is approximately 146 kDa which is typical for IgG molecules. IgG4 is commonly expressed in the blood and extracellular fluids. It can also be found at sites of chronic inflammation reflecting its involvement in modulating immune responses.

Biological function summary

IgG4 antibodies act to regulate immune responses and prevent excessive inflammation. It does not engage in strong effector functions like other IgG subclasses due to its unique ability to exchange half-molecules leading to functional monovalency. This characteristic makes it part of an immune complex that can block other antibody functions by competitive binding without activating complement pathways. The IgG4 sequence shows unique properties that allow it to engage selectively in regulatory pathways which is a distinct attribute among immunoglobulins.

Pathways

IgG4 functions within immune regulatory pathways that inhibit inflammation. It involves pathways like the Fc gamma receptor-mediated suppression of effector cell responses. Through this pathway IgG4 can interact with proteins such as Fc gamma receptors on cells altering signaling cascades that prevent unnecessary immune activation. IgG4 antibodies play these roles alongside other immune-modulating proteins helping to balance immune system activity.

IgG4 relates to conditions like IgG4-related disease (IgG4-RD) and autoimmune diseases where it presents an abnormal increase in serum levels and tissue infiltration. IgG4-RD is characterized by elevated IgG4 protein and causes fibrotic tissue inflammation in organs such as the pancreas and salivary glands. IgG4's interaction with proteins like T regulatory cells may contribute to downregulating immune attacks in autoimmune disease highlighting its dual role in both defensive and regulatory immune activities.

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产品实验方案

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靶点信息

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region interacts (via the fragment crystallizable, Fc) with the Fc receptors on innate immune cells to mediate humoral effector functions. Ig gamma-4 (IgG4) isotype does not elicit antibody-dependent cellular cytotoxicity (ADDC) or complement-dependent cytotoxicity (CDC). Instead it is likely involved in immune tolerance mechanisms to allergens and parasites either by blocking IgE-antigen complex formation or by directly inhibiting mast cell degranulation through Fc receptor signaling. In the context of tumorigenesis, it may participate in immunosuppressive mechanisms.

See full target information IGHG4

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websiteProtocolBooklet

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Human IgG4 ELISA Kit