人IGFBP2 ELISA试剂盒(ab272207)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 115.38 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IGFBP2 ELISA试剂盒
参阅全部 IGFBP2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 2.7% 批次间 样品 n Mean SD CV% Serum 3 3.2% -
样品类型
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
115.38 pg/ml -
范围
312.5 pg/ml - 20000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 104 97% - 108% Urine 86 85% - 89% Serum 94 92% - 98% Hep Plasma 93 89% - 98% EDTA Plasma 94 91% - 97% Cit plasma 88 82% - 95% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human IGFBP2 ELISA kit (ab272207) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP2 protein in serum, plasma, urine and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGFBP2 with 115.38 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Insulin-like growth factor-binding protein 2 (IGFBP2) is an IGF binding protein involved in prolonging its half-life and has both inhibitory and stimulatory effects on IGFs. IGFBP2 can also alter the interaction of IGFs with their receptors.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human IGFBP2 Capture Antibody 1 x 600µl 10X Human IGFBP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IGFBP2 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
序列相似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
结构域
The C-terminus is required for IGF-binding and growth inhibition. -
翻译后修饰
O-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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别名
- BP 2
- BP2
- IBP 2
see all -
数据库链接
- Entrez Gene: 3485 Human
- Omim: 146731 Human
- SwissProt: P18065 Human
- Unigene: 438102 Human
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272207于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IGFBP2 competitor curve comparisonStandard Curve comparison between Human IGBPF2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.
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Human IGFBP2 standard curve comparisonStandard Curve comparison between the original Human IGFBP2 SimpleStep ELISA kit (ab215082) and current Human MMP3 SimpleStep ELISA kit (ab272207).
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Example of human IGFBP2 standard curve in Sample Diluent NS.The IGFBP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IGFBP2 in human serum and plasma samples.The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 257.87 ng/mL in serum, 273.81 ng/mL in plasma (citrate), 139.86 ng/ml in plasma (heparin), and 118.43 ng/ml in plasma (EDTA).
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Interpolated concentrations of native IGFBP2 in human PBMC cell culture supernatant samples treated for 46 hours with 1.5% PHA-M.The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 3,034.42 pg/mL in PBMC supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate. -
Sandwich ELISA - Human IGFBP2 ELISA Kit (ab272207)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
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