人IgA1 ELISA试剂盒
Human IgA1 ELISA Kit
- SimpleStep
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Human IgA1 ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human IgA1 with a sensitivity of 0.12 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab326405
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Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1
- sELISA
Supplier Data
Sandwich ELISA - Human IgA1 ELISA Kit (AB316261)
Interpolated concentration of native IgA1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 1 : 100, milk 1 : 20,000, and saliva 1 : 2,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human IgA1 ELISA Kit (AB316261)
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgA1 concentration was determined to be 1,218 µg/mL with a range of 639 – 2,204 µg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human IgA1 ELISA Kit (AB316261)
Interpolated concentration of native IgA1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 62,500, plasma (citrate) 1 : 50,000, plasma (EDTA) 1 : 50,000, and plasma (heparin) 1 : 100,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human IgA1 ELISA Kit (AB316261)
Example of human IgA1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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反应性数据
产品详情
Human IgA1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgA1 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Urine, Milk, Saliva, Cell culture supernatant. Quantitate Human IgA1 with 0.12 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.
Pathways
IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.
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