人IFN gamma ELISA试剂盒(ab174443)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 470 pg/ml
- Range: 0.468 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IFN gamma ELISA试剂盒
参阅全部 Interferon gamma 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5 1.1% 批次间 样品 n Mean SD CV% Overall 3 7.9% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
470 pg/ml -
范围
0.468 ng/ml - 30 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 102 98% - 104% Cell culture media 86 77% - 93% Hep Plasma 244 197% - 298% EDTA Plasma 122 117% - 124% Cit plasma 107 96% - 115% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human IFN gamma ELISA kit (ab174443) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IFN-gamma protein in human cell culture supernatant, plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IFN gamma with 470 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human Interferon-gamma protein in serum, plasma, and cell culture supernatant samples only.
Urine, milk, and cell and tissue extract samples have not been tested with this kit.
ASSAY INTERFERENCE
This kit is incompatible with plasma (heparin) samples.
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说明
IFN gamma (IFNG) is produced by lymphocytes activated by specific antigens or mitogens. IFN gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 384 tests 1 x 96 tests 10X Human IFNG Capture Antibody 1 x 600µl 1 x 600µl 10X Human IFNG Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 1 unit 0 x 0 unit Antibody Diluent CPI2 1 x 6ml 1 x 6ml Human IFNG Lyophilized Recombinant Protein 2 vials 2 vials Plate Seal 1 unit 1 unit Sample Diluent NBP 1 x 20ml 1 x 20ml Sample Diluent NS (ab193972) 1 x 100ml 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 0 x 0 unit 1 unit Stop Solution 2 x 12ml 1 x 12ml TMB Development Solution 2 x 12ml 1 x 12ml -
研究领域
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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别名
- IF 1
- IFG
- IFI
see all -
数据库链接
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
相关产品
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ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174443于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example IFNG standard curve for cell culture supernatant samples measurements.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Interferon-gamma standard curve in Sample Diluent NS (for supernatant samples)The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example IFNG standard curve for serum/plasma samples measurements.Background-subtracted data values (mean +/- SD) are graphed. -
Example of human Interferon-gamma standard curve in Sample Diluent NBP (for serum/plasma samples)The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Comparison of secreted IFNG in unstimulated and PHA-stimulated Human PBMC.PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 2 days. IFNG concentrations were measured in 12X and 6X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC, and media. Raw data values (mean +/-SD, n=3) are graphed. The dotted line represents zero sample background. -
Interpolated concentrations of secreted IFNG in unstimulated and PHA-stimulated Human PBMC.The concentrations of IFNG were interpolated from data values shown in Figure 3 using IFNG standard curve and corrected for sample dilution. The mean IFNG concentration was determined to be 1.8 ng/mL in unstimulated PBMC supernatants and 177.2 ng/mL in stimulated PBMC supernatants. -
Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Interferon-gamma was measured in PHA-stimulated PBMC cell culture supernatant samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Recombinant Interferon-gamma was spiked into human serum and plasma (citrate and EDTA) samples and diluted in a 2-fold dilution series in Sample Diluent NBP.Neat pooled serum and plasma (EDTA and Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
Neat serum from ten individual healthy human female/male donors was measured in duplicate. All values were below the detectable range of the assay.
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Example of human IFN-gamma standard curve in Sample Diluent NBP in 96-well vs. 384-well plate.Example of human IFN-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IFN-gamma standard curve in Sample Diluent NS in 96-well vs. 384-well plate.Example of human IFN-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human IFN-gamma in stimulated PBMC supernatant in 96-well vs. 384-well plates.Interpolated concentration of native IFN-gamma was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20% treated human PBMC supernatant (5% PHA-M for 46hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab174443 被引用在 26 文献中.
- Sato O et al. Tumor Growth Suppression of Pancreatic Cancer Orthotopic Xenograft Model by CEA-Targeting CAR-T Cells. Cancers (Basel) 15:N/A (2023). PubMed: 36765558
- Yang G & Qiu Y Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial. Medicine (Baltimore) 102:e32384 (2023). PubMed: 36820569
- Ding L et al. Midkine inhibition enhances anti-PD-1 immunotherapy in sorafenib-treated hepatocellular carcinoma via preventing immunosuppressive MDSCs infiltration. Cell Death Discov 9:92 (2023). PubMed: 36906597
- Hu Y et al. Predictive Values of Blood Type I and Type II Interferon Production for Disease Activity and Clinical Response to TNF-α Blocking Therapy in Patients with Ankylosing Spondylitis. Tohoku J Exp Med 260:263-271 (2023). PubMed: 37081619
- Zeng X et al. RNF182 induces p65 ubiquitination to affect PDL1 transcription and suppress immune evasion in lung adenocarcinoma. Immun Inflamm Dis 11:e864 (2023). PubMed: 37249301