JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174443

人IFN gamma ELISA试剂盒

Human IFN gamma ELISA Kit

Be the first to review this product! Submit a review

|

(26 Publications)

Human IFN gamma (IFNG) ELISA kit is a single-wash, 90-minute SimpleStep ELISA® a used to quantify human interferon-gamma (IFN-γ) with a sensitivity of 470 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader
- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput
- Broad sample compatibility – suitable for serum, plasma (citrate, EDTA), and cell culture supernatants
- Cited in over 25 publications

查看别名

Interferon gamma, IFN-gamma, Immune interferon, IFNG

11 Images
ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)
ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)

Example IFNG standard curve for cell culture supernatant samples measurements. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Interpolated concentration of native IFN-gamma was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20% treated human PBMC supernatant (5% PHA-M for 46hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Interferon-gamma was measured in PHA-stimulated PBMC cell culture supernatant samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Recombinant Interferon-gamma was spiked into human serum and plasma (citrate and EDTA) samples and diluted in a 2-fold dilution series in Sample Diluent NBP.

Neat pooled serum and plasma (EDTA and Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

Neat serum from ten individual healthy human female/male donors was measured in duplicate. All values were below the detectable range of the assay.

ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)

Example IFNG standard curve for serum/plasma samples measurements. Background-subtracted data values (mean +/- SD) are graphed.

ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)

Interpolated concentrations of secreted IFNG in unstimulated and PHA-stimulated Human PBMC. The concentrations of IFNG were interpolated from data values shown in Figure 3 using IFNG standard curve and corrected for sample dilution. The mean IFNG concentration was determined to be 1.8 ng/mL in unstimulated PBMC supernatants and 177.2 ng/mL in stimulated PBMC supernatants.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Example of human IFN-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)

Comparison of secreted IFNG in unstimulated and PHA-stimulated Human PBMC. PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 2 days. IFNG concentrations were measured in 12X and 6X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC, and media. Raw data values (mean +/-SD, n=3) are graphed. The dotted line represents zero sample background.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Example of human IFN-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Example of human Interferon-gamma standard curve in Sample Diluent NS (for supernatant samples).

The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Example of human Interferon-gamma standard curve in Sample Diluent NBP (for serum/plasma samples).

The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 470 pg/mL

范围

0.468 - 30 ng/mL

检测时间

1h 30m

检测平台

Microplate

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

产品详情

Human IFN gamma ELISA Kit ab174443 is a rapid single-wash 90-min ELISA kit to measure Human IFN gamma in cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 470 pg/mL.

How the assay works

Human IFN gamma SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This IFN-γ ELISA Kit recognizes both native and recombinant human Interferon-gamma protein in serum, plasma, and cell culture supernatant samples only. Urine, milk, and cell and tissue extract samples have not been tested with this kit.

Human IFN gamma ELISA Kit ab174443 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IFN gamma ELISA Kit ab174443

Human IFN gamma ELISA Kit ab174443 has been used to study inflammation in different physio-pathological contexts such as celiac disease(1), pancreatic cancer(2)and atherosclerosis(3).

References:

(1)Soroush Sardari et al. 2023, PMID: 38040898,
(2)Satoshi Hirano et al. 2023, PMID: 36765558,
(3)Youjiang Qiu, et al. 2023, PMID: 36820569

Save your precious samples

Our 384-well Human IFN gamma ELISA Kit only requires a maxmum of 12.5µL

Related and recommended products

Human IFN gamma ELISA Kit ab174443 is commonly used to measure IFNg ELISA as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Human IL-6 ELISA kit ab178013
- Human TNF alpha ELISA kit ab181421
- Human IL-1 2 ELISA kit ab270883

To measure IFN gamma at a lower dynamic range (12.5-1600 pg/mL):

- Human IFN gamma High Sensitivity ELISA kit ab236895

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.1" } ]
style
left-column

回收率

[ { "sample": "Serum", "range": "98 - 104 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "77 - 93 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "96 - 115 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "117 - 124 %", "average": "= 122" }, { "sample": "Heparin Plasma", "range": "197 - 298 %", "average": "= 244" } ]
style
left-column

规格

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab174443-1x384Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human IFNG Lyophilized Recombinant Protein", "number":"AB174443-CMP03" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB174443-CMP13" }, { "size":"1 x 100 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB174443-CMP12" }, { "size":"1 x 600 µL", "name":"10X Human IFNG Capture Antibody", "number":"AB174443-CMP08" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB174443-CMP11" }, { "size":"1 x 20 mL", "name":"Sample Diluent NBP", "number":"AB174443-CMP04" }, { "size":"1 x 600 µL", "name":"10X Human IFNG Detector Antibody", "number":"AB174443-CMP09" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB174443-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB174443-CMP07" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB174443-CMP10" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB174443-CMP14" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab174443-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB174443-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB174443-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent NBP", "number":"AB174443-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IFNG Capture Antibody", "number":"AB174443-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB174443-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB174443-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IFNG Lyophilized Recombinant Protein", "number":"AB174443-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB174443-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB174443-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB174443-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IFNG Detector Antibody", "number":"AB174443-CMP09", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naпve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG
style
left-column

文献 (26)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadj9964 PubMed38134285

2023

Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoyu Zhong,Matthew Sullivan,Neil Upreti,Roy Chen,Agustin De Ganzó,Kaichun Yang,Shujie Yang,Ke Jin,Ye He,Ke Li,Jianping Xia,Zhiteng Ma,Luke P Lee,Tania Konry,Tony Jun Huang

Scientific reports 13:21180 PubMed38040898

2023

Bromelain-loaded nanocomposites decrease inflammatory and cytotoxicity effects of gliadin on Caco-2 cells and peripheral blood mononuclear cells of celiac patients.

Applications

Unspecified application

Species

Unspecified reactive species

Masoumeh Sadat Mousavi Maleki,Ramin Ebrahimi Kiasari,Seyed Javad Seyed Mousavi,Hamid Hashemi-Moghaddam,Ali Akbar Shabani,Hamid Madanchi,Soroush Sardari

Nature communications 14:7639 PubMed37993431

2023

Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Ye He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun Huang

Clinical and translational medicine 13:e1410 PubMed37712124

2023

Long noncoding RNA Regulating ImMune Escape regulates mixed lineage leukaemia protein-1-H3K4me3-mediated immune escape in oesophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Liu,Wei-Yi Zhou,Xiao-Jing Luo,Yan-Xing Chen,Chau-Wei Wong,Ze-Xian Liu,Jia- Bo Zheng,Hai- Yu Mo,Jun-Quan Chen,Jia-Jun Li,Ming Zhong,Yu-Hong Xu,Qi-Hua Zhang,Heng-Ying Pu,Qi-Nian Wu,Ying Jin,Zi-Xian Wang,Rui-Hua Xu,Hui-Yan Luo

Biological & pharmaceutical bulletin 46:1260-1268 PubMed37661405

2023

Cordycepin Enhances the Cytotoxicity of Human Natural Killer Cells against Cancerous Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nipha Chaicharoenaudomrung,Phongsakorn Kunhorm,Parinya Noisa

Immunity, inflammation and disease 11:e864 PubMed37249301

2023

RNF182 induces p65 ubiquitination to affect PDL1 transcription and suppress immune evasion in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xingdu Zeng,Xiaoyuan Tang,Xingxiang Chen,Huilan Wen

The Tohoku journal of experimental medicine 260:263-271 PubMed37081619

2023

Predictive Values of Blood Type I and Type II Interferon Production for Disease Activity and Clinical Response to TNF-α Blocking Therapy in Patients with Ankylosing Spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yulan Hu,Bo Lou,Zhonghua Jiang,Chunchu Yu

Cell death discovery 9:92 PubMed36906597

2023

Midkine inhibition enhances anti-PD-1 immunotherapy in sorafenib-treated hepatocellular carcinoma via preventing immunosuppressive MDSCs infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Ding,Nanya Wang,Qiang Wang,Xia Fan,Yuning Xin,Shudong Wang

Medicine 102:e32384 PubMed36820569

2023

Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Gui Yang,Youjiang Qiu

Cancers 15: PubMed36765558

2023

Tumor Growth Suppression of Pancreatic Cancer Orthotopic Xenograft Model by CEA-Targeting CAR-T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Osamu Sato,Takahiro Tsuchikawa,Takuma Kato,Yasunori Amaishi,Sachiko Okamoto,Junichi Mineno,Yuta Takeuchi,Katsunori Sasaki,Toru Nakamura,Kazufumi Umemoto,Tomohiro Suzuki,Linan Wang,Yizheng Wang,Kanako C Hatanaka,Tomoko Mitsuhashi,Yutaka Hatanaka,Hiroshi Shiku,Satoshi Hirano
View all publications
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
websiteProtocolBooklet
en
style
right-column

关联产品

Select an associated product type
Alternative Product
Assay Kits

AB236895

Human IFN gamma High Sensitivity ELISA Kit

elisa-kits

human-ifn-gamma-high-sensitivity-elisa-kit-ab236895

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com