人ICOS Ligand ELISA试剂盒(B7-H2) (ab272475)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21.8 pg/ml
- Range: 109 pg/ml - 7000 pg/ml
- Sample type: Cell culture media, Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人ICOS Ligand ELISA试剂盒(B7-H2)
参阅全部 ICOS Ligand/ICOSL 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Extract 8 3.9% 批次间 样品 n Mean SD CV% Extract 4 4.9% -
样品类型
Cell Lysate, Cell culture media -
检测类型
Sandwich (quantitative) -
灵敏度
21.8 pg/ml -
范围
109 pg/ml - 7000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell Lysate 107 102% - 111% Cell culture media 100 94% - 104% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human ICOS Ligand ELISA kit (ab272475) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ICOS Ligand protein in cell extract and culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ICOS Ligand with 21.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Inducible T Cell Co-Stimulator Ligand (ICOSLG), also known as CD275 and B7-H2, is a glycoprotein encoded by the gene ICOSLG. ICOS Ligand is a single pass type I membrane protein, which consists of a 238 -amino acid extracellular portion, a 21- amino acid transmembrane region, and a 25-amino acid cytoplasmic region. ICOS Ligand is constitutively expressed in many tissues and is enhanced by treatment with TNF in peripheral blood B-cells and monocytes. ICOS acts as a costimulatory signal for T-cell proliferation and cytokine secretion. ICOS Ligand induces B-Cell proliferation and differentiation into plasma cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human ICOS Ligand (B7-H2) Capture Antibody 1 x 600µl 10X Human ICOS Ligand (B7-H2) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI2 1 x 6ml Human ICOS Ligand (B7-H2) Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. -
组织特异性
Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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别名
- B7 H2
- B7 homolog 2
- B7 homologue 2
see all -
数据库链接
- Entrez Gene: 23308 Human
- Omim: 605717 Human
- SwissProt: O75144 Human
- Unigene: 14155 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272475于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of human ICOS Ligand standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
The ICOS Ligand standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ICOS Ligand in human HEK293 overexpression extract based on a 5 µg/mL extract load.The concentrations of ICOS Ligand were measured in duplicate and interpolated from the ICOS Ligand standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human ICOS Ligand ELISA Kit (B7-H2) (ab272475)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human ICOS Ligand ELISA Kit (B7-H2) (ab272475)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Human ICOS Ligand ELISA Kit (B7-H2) (ab272475)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272475 被引用在 1 文献中.
- Fukasawa T et al. Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model. Elife 10:N/A (2021). PubMed: 34854378