人Anti-HSP60 IgG/A/M ELISA试剂盒(ab133059)
Key features and details
- Sensitivity: 2.88 ng/ml
- Range: 7.81 ng/ml - 250 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
概述
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产品名称
人Anti-HSP60 IgG/A/M ELISA试剂盒
参阅全部 Hsp60 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 16 < 10% 批次间 样品 n Mean SD CV% Overall 16 < 10% -
样品类型
Serum -
检测类型
Indirect -
灵敏度
2.88 ng/ml -
范围
7.81 ng/ml - 250 ng/ml -
检测时间
1h 15m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human Anti-HSP60 IgG/A/M in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Anti-HSP60 in Human Serum. This assay allows determination of IgG, IgA and IgM antibodies (total) to Human Hsp60 in serum.
A recombinant Human Hsp60 protein has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. An anti-Human GAM-HRP conjugated antibody is then added and incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
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说明
HSP60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. HSP60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of HSP60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.
Plates are not provided
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经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Anti-Human GAM-HRP Conjugate 1 x 10ml Human Anti-HSP60 IgG/A/M Microplate (12 x 8 wells) 1 unit Human Anti-HSP60 Standard 1 x 0.12ml Plate Sealer 3 units Sample Diluent 1 1 x 100ml Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
研究领域
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功能
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
疾病相关
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
序列相似性
Belongs to the chaperonin (HSP60) family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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别名
- 60 kDa chaperonin
- 60 kDa heat shock protein, mitochondrial
- CH60_HUMAN
see all -
数据库链接
- Entrez Gene: 3329 Human
- Omim: 118190 Human
- SwissProt: P10809 Human
- Unigene: 595053 Human
- Unigene: 727543 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133059于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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