人HSP60 ELISA试剂盒(ab124534)
Key features and details
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人HSP60 ELISA试剂盒
参阅全部 HSP60 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4 2.1% 批次间 样品 n Mean SD CV% Overall 3 6.7% -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Sandwich (quantitative) -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
产品概述
ab124534 HSP60 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of HSP60 protein a 60 kDa protein encoded by the Human HSPD1 gene, in cell and tissue lysates. The assay employs an HSP60 specific antibody coated onto well plate strips.
Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-HSP60 primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Species– Human reactive. Non-reactive with mouse and rat. Others untested.
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说明
Typical working ranges:
HepG2 cell extract: 0.39 - 200 µg/mL
Hela cell extract: 3.125 - 200 µg/mL
Store all components at 4°C. This kit is stable for at least 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 10X HSP60 Detector Antibody 1 x 1ml 20X Buffer 1 x 20ml Extraction Buffer (ab260490) 1 x 15ml HSP60 Microplate 1 unit HRP Development Solution 1 x 12ml -
研究领域
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别名
- Chaperonin
- Heat shock 60kDa protein 1
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数据库链接
- Entrez Gene: 3329 Human
- SwissProt: P10809 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124534于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Flow Cytometry - HSP60 Human ELISA Kit (ab124534)Analysis of HSP60 staining in Hela cells by Flow: blue: 1µg/mL HSP60 antibody; red: an equal amount of an isotype control antibody.
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Immunocytochemistry/ Immunofluorescence - HSP60 Human ELISA Kit (ab124534)Analysis of HSP60 staining in HDFn cells by ICC: HSP60 primary antibody at 1 µg/mL (green). -
Western blot - HSP60 Human ELISA Kit (ab124534)Western blot using the HSP60 detector antibody at 1µg/mL demonstrates assay specificity. HepG2 cell lysate loaded at 15µg/lane. -
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)Sensitivity of HSP60 ELISA kit is used to demonstrate the quantitative differentiation among different human cell lines. -
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)Detection of endogenous HSP60 levels in different cell lysates using HSP60 ELISA kit. -
Western blot - HSP60 Human ELISA Kit (ab124534)Detection of endogenous HSP60 levels in different cell lysates using Western blot. HSP60 antibody at 1µg/mL: HepG2 cell lysate (lane 2), HeLa cell lysate (lane 3), and HDFn lysate (lane 4), each were loaded at 15µg/lane. -
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)Example standard curve of serially titrated Hela cell lysate in the working range of the assay. -
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)Example standard curve of serially titrated HepG2 cell lysate in the working range of the assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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