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AB171577

人HIF-1 alpha ELISA试剂盒

Human HIF-1 alpha ELISA Kit

3

(1 Review)

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(18 Publications)

Human HIF-1 alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HIF-1 alpha with a sensitivity of 42 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 15 citations

查看别名

BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78

6 Images
ELISA - Human HIF-1 alpha ELISA Kit (AB171577)
  • ELISA

Supplier Data

ELISA - Human HIF-1 alpha ELISA Kit (AB171577)
ELISA - Human HIF-1 alpha ELISA Kit (AB171577)
  • ELISA

Unknown

ELISA - Human HIF-1 alpha ELISA Kit (AB171577)

Comparison of HIF1 alpha expression in HeLa cell extracts (with and without DFO treatment) by ELISA (barchart) and western blot (top). Background subtracted OD450 nm data from three loading concentrations are shown. The HIF1 alpha detector antibody was used to blot the same lysates as analyzed by ELISA (40 μg loaded/lane). The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, DFO treatment is required to detect HIF1 alpha protein by both ELISA and western blot.

ELISA - Human HIF-1 alpha ELISA Kit (AB171577)
  • ELISA

Unknown

ELISA - Human HIF-1 alpha ELISA Kit (AB171577)

Titration of HeLa-DFO (ab120727) extract within the working range of the assay.

Titration of HeLa-DFO extract within the working range of the assay. Background subtracted data from duplicate measurements are plotted. To induce HIF1 alpha protein levels, HeLa cells were treated with 500 μM Deferoxamine (DFO) for 24 hours.

ELISA - Human HIF-1 alpha ELISA Kit (AB171577)
  • ELISA

Unknown

ELISA - Human HIF-1 alpha ELISA Kit (AB171577)

Example HIF1 alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human HIF-1 alpha ELISA Kit (AB171577)
  • sELISA

Supplier Data

Sandwich ELISA - Human HIF-1 alpha ELISA Kit (AB171577)

Representative image of Human HIF-1 alpha ELISA Kit (ab171577)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human HIF-1 alpha Capture Antibody

- 10X Human HIF-1 alpha Detector Antibody

- Human HIF-1 alpha Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5B

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

ELISA - Human HIF-1 alpha ELISA Kit (AB171577)
  • ELISA

Unknown

ELISA - Human HIF-1 alpha ELISA Kit (AB171577)

Dose-dependent induction of HIF1 alpha in HeLa cells by DFO (ab120727).

Dose-dependent induction of HIF1 alpha in HeLa cells by deferoxamine (DFO). HeLa cells were cultured in 96-well tissue culture plates and were either untreated or exposed to varying dose of DFO for 24 hours. Raw data with standard deviation is plotted from triplicate measurements.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 42 pg/mL

范围

0.23 - 15 ng/mL

检测时间

1h 30m

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human HIF-1 alpha ELISA Kit ab171577 is a rapid single-wash 90-min Sandwich ELISA to measure Human HIF-1 alpha in cell culture extracts. This SimpleStep sensitivity is 42 pg/mL.

How the assay works

Human HIF-1 alpha SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human HIF-1 alpha ELISA Kit ab171577 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
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精确度

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]
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回收率

[ { "sample": "Bovine Serum Albumin", "range": "93 - 106 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "94 - 129 %", "average": "= 112" }, { "sample": "Fetal Bovine Serum", "range": "102 - 120 %", "average": "= 109" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171577-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB171577-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB171577-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB171577-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB171577-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB171577-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB171577-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HIF1a Capture Antibody", "number":"AB171577-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5B", "number":"AB171577-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB171577-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HIF1a Detector Antibody", "number":"AB171577-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"HIF1a Human Lyophilized Protein", "number":"AB171577-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB171577-CMP05", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
Biological function summary

HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.

Pathways

HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.

HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.
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产品实验方案

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靶点信息

The protein expressed by the gene HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia, activating the transcription of over 40 genes under hypoxic conditions, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and others. These genes' protein products enhance oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A is crucial for embryonic vascularization, tumor angiogenesis, and ischemic disease pathophysiology. Its activation requires transcriptional coactivators like CREBBP and EP300, with activity enhanced by interactions with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 activates CTAD and enhances activation by NCOA1 and CREBBP. Additionally, HIF1A is involved in axonal distribution and mitochondrial transport in neurons during hypoxia. In the context of microbial infection, specifically human coronavirus SARS-CoV-2, HIF1A is necessary for glycolysis induction in monocytes, leading to a proinflammatory state, inducing expression of ACE2, cytokines, and promoting virus replication and monocyte inflammatory response. This supplementary information is collated from multiple sources and compiled automatically.
See full target information HIF1A
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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Toxics 11: PubMed36851057

2023

A Proposed Association between Improving Energy Metabolism of HepG2 Cells by Plant Extracts and Increasing Their Sensitivity to Doxorubicin.

Applications

Unspecified application

Species

Unspecified reactive species

Tamer A Al-Shafie,Engy A Mahrous,Mustafa Shukry,Mohammad Y Alshahrani,Samah F Ibrahim,Liana Fericean,Afaf Abdelkader,Mennatallah A Ali

Frontiers in nutrition 9:857256 PubMed35464036

2022

Carvacrol Arrests the Proliferation of Hypopharyngeal Carcinoma Cells by Suppressing Ornithine Decarboxylase and Hyaluronidase Activities.

Applications

Unspecified application

Species

Unspecified reactive species

Kaneez Fatima,Suaib Luqman,Abha Meena

Iranian journal of pharmaceutical research : IJPR 20:316-332 PubMed34567165

2021

Inhibition of HMGB1 Might Enhance the Protective Effect of Taxifolin in Cardiomyocytes via PI3K/AKT Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Erjun Feng,Jian Wang,Xinwei Wang,Zhenguo Wang,Xiaochu Chen,Xu Zhu,Wenli Hou

International journal of stem cells 14:221-228 PubMed33632987

2021

Optimal Hypoxic Preconditioning of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells (hES-MSCs) and Their Characteristics.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Min Lee,Dae Won Jun,Hyeon Tae Kang,Ju Hee Oh,Waqar Khalid Saeed,Sang Bong Ahn

Journal of gastroenterology and hepatology 36:1997-2007 PubMed33554346

2021

Macro-encapsulation of mesenchymal stem cells in acute and chronic liver injury animal models.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeon Tae Kang,Kiseok Jang,Dae Won Jun,Eileen L Yoon,Seung Min Lee,Waqar Khalid Saeed,Jin Ho Lee

International journal of molecular sciences 21: PubMed33143089

2020

Hypoxia Regulates DPP4 Expression, Proteolytic Inactivation, and Shedding from Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laura R Moffitt,Maree Bilandzic,Amy L Wilson,Yiqian Chen,Mark D Gorrell,Martin K Oehler,Magdalena Plebanski,Andrew N Stephens

Experimental and therapeutic medicine 20:141 PubMed33093879

2020

Expression and predictive value of HIF-1α and VEGF in patients with burns following treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhufeng Liu,Ling Liu,Xuejuan Cheng,Liming Gao

FEBS letters 595:811-827 PubMed32978975

2020

Low oxygen tension differentially regulates the expression of placental solute carriers and ABC transporters.

Applications

Unspecified application

Species

Unspecified reactive species

Ludwik Gorczyca,Jianyao Du,Kristin M Bircsak,Xia Wen,Anna M Vetrano,Lauren M Aleksunes

BioMed research international 2020:1587915 PubMed32884936

2020

Association of Nuclear Receptor Coactivators with Hypoxia-Inducible Factor-1 in the Serum of Patients with Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tianbiao Zhou,Wenshan Lin,Shujun Lin,Zhiqing Zhong,Yuanyuan Luo,Zhijun Lin,Weiji Xie,Weitao Shen,Kai Hong

Cancers 12: PubMed32707933

2020

Chemically Induced Hypoxia Enhances miRNA Functions in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Gervin,Bonita Shin,Reid Opperman,Mackenzie Cullen,Riley Feser,Sujit Maiti,Mousumi Majumder
View all publications
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