人Anti-Herpes simplex virus Type 2 IgG ELISA试剂盒(HSV2) (ab108739)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
概述
-
产品名称
人Anti-Herpes simplex virus Type 2 IgG ELISA试剂盒(HSV2) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Pos.Serum 20 3.4% Pos.Serum 24 3.7% 批次间 样品 n Mean SD CV% Pos.Serum 12 3.6% Pos.Serum 12 5.1% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Indirect -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s anti-Herpes simplex virus Type 2 (HSV2) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Herpes simplex virus Type 2 in Human serum and plasma.
A 96-well plate has been precoated with Herpes simplex virus Type 2 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Herpes simplex virus Type 2-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Herpes simplex virus Type 2 IgG sample captured in plate.
-
经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Herpes simplex virus Type 2 (IgG) Coated Microplate (12 x 8 wells) 1 unit Herpes simplex virus Type 2 anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Herpes simplex virus Type 2 IgG Cut-off Control Yellow, Green cap 1 x 3ml Herpes simplex virus Type 2 IgG Negative Control Yellow, Blue cap 1 x 2ml Herpes simplex virus Type 2 IgG Positive Control Yellow, Red cap 1 x 2ml IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
相关产品
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108739于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
-
Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab108739 被引用在 2 文献中.
- Salovin A et al. Anti-NMDA receptor encephalitis and nonencephalitic HSV-1 infection. Neurol Neuroimmunol Neuroinflamm 5:e458 (2018). PubMed: 29629396
- Slawinski BL et al. Maternal cytomegalovirus sero-positivity and autism symptoms in children. Am J Reprod Immunol 79:e12840 (2018). PubMed: 29520885