人Hemopexin ELISA试剂盒(HPX) (ab108860)
Key features and details
- Sensitivity: 1.3 ng/ml
- Range: 12.5 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Hemopexin ELISA试剂盒(HPX)
参阅全部 Hemopexin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.3% 批次间 样品 n Mean SD CV% Overall 9.5% -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 1.3 ng/ml -
范围
12.5 ng/ml - 200 ng/ml -
回收率
98 %
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检测时间
3h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Hemopexin (HPX) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemopexin levels in Human cell culture supernatants, milk, saliva and urine.
A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Hemopexin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Hemopexin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Hemopexin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Hemopexin Microplate (12 x 8 well strips) 1 unit Hemopexin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
组织特异性
Expressed by the liver and secreted in plasma. -
序列相似性
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
翻译后修饰
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
细胞定位
Secreted. - Information by UniProt
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别名
- Beta 1B glycoprotein
- Beta-1B-glycoprotein
- FLJ56652
see all -
数据库链接
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108860于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108860.
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Sandwich ELISA - Hemopexin (HPX) Human ELISA Kit (ab108860)Hemopexin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human saliva diluted 60-120 fold. Human milk diluted 300-600 fold. Human urine diluted 10 fold - below detection. Cell supernatants tested neat.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab108860 被引用在 2 文献中.
- Zheng W et al. Cerebrospinal Fluid Proteins Identification Facilitates the Differential Diagnosis of Central Nervous System Diffuse Large B Cell Lymphoma. J Cancer 8:3631-3640 (2017). Functional Studies . PubMed: 29151949
- Zager RA et al. Renal cortical hemopexin accumulation in response to acute kidney injury. Am J Physiol Renal Physiol 303:F1460-72 (2012). Sandwich ELISA . PubMed: 22993068