人Hemopexin ELISA试剂盒(HPX) (ab108859)
Key features and details
- Sensitivity: 73 ng/ml
- Range: 0.078 µg/ml - 20 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
人Hemopexin ELISA试剂盒(HPX)
参阅全部 Hemopexin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3.9% 批次间 样品 n Mean SD CV% Overall 10.3% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
> 73 ng/ml -
范围
0.078 µg/ml - 20 µg/ml -
回收率
95 %
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检测时间
2h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.
A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.
Get results in 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 2X Biotinylated Human Hemopexin (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Hemopexin Microplate (12 x 8 well strips) 1 unit Hemopexin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
组织特异性
Expressed by the liver and secreted in plasma. -
序列相似性
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
翻译后修饰
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
细胞定位
Secreted. - Information by UniProt
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别名
- Beta 1B glycoprotein
- Beta-1B-glycoprotein
- FLJ56652
see all -
数据库链接
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108859于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108859.
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Competitive ELISA - Hemopexin (ab108859Hemopexin measured in various biological fluids showing quantity (μg) per mL of tested sample
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab108859 被引用在 7 文献中.
- Meegan JE et al. Transpulmonary generation of cell-free hemoglobin contributes to vascular dysfunction in pulmonary arterial hypertension via dysregulated clearance mechanisms. Pulm Circ 13:e12185 (2023). PubMed: 36743426
- Liu J et al. Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice. Sci Adv 8:eadc9245 (2022). PubMed: 36563141
- Paul S et al. Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans. Funct Integr Genomics 20:191-200 (2020). PubMed: 31444657
- Canesin G et al. Scavenging of Labile Heme by Hemopexin Is a Key Checkpoint in Cancer Growth and Metastases. Cell Rep 32:108181 (2020). PubMed: 32966797
- Lawson HA et al. Physiologic and genetic evidence links hemopexin to triglycerides in mice and humans. Int J Obes (Lond) 41:631-638 (2017). PubMed: 28119529
- Evans C et al. Impairment of neutrophil oxidative burst in children with sickle cell disease is associated with heme oxygenase-1. Haematologica N/A:N/A (2015). PubMed: 26315932
- Insenser M et al. Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: a nontargeted proteomic study. PLoS One 9:e100902 (2014). PubMed: 24979072