人Heat Shock蛋白27 ELISA试剂盒(HSP27) (ab108862)
Key features and details
- Sensitivity: 0.16 ng/ml
- Range: 1.25 ng/ml - 20 ng/ml
- Sample type: Cell Lysate, Milk, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Heat Shock蛋白27 ELISA试剂盒(HSP27)
参阅全部 Heat Shock Protein 27 (HSP27) 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4.8% 批次间 样品 n Mean SD CV% Overall 9.9% -
样品类型
Milk, Serum, Plasma, Tissue, Cell Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
> 0.16 ng/ml -
范围
1.25 ng/ml - 20 ng/ml -
回收率
97 %
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检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Heat Shock Protein 27 (HSP27) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP27 levels in cell culture samples, milk, serum, plasma and tissue extract.
A HSP27 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP27 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP27 captured in plate.
Get results in 90 minutes with Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab193757) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human HSP27 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml HSP27 Microplate (12 x 8 well strips) 1 unit HSP27 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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相关性
Involved in stress resistance and actin organization. Interacts with TGFB1I1. Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1. -
细胞定位
Cytoplasm. Nucleus. Cytoplasm; cytoskeleton; spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. -
别名
- HSPB1
- 28 kDa heat shock protein
- CMT2F
see all -
数据库链接
- Entrez Gene: 3315 Human
- Omim: 602195 Human
- SwissProt: P04792 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108862于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108862.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108862 被引用在 5 文献中.
- Zhou XY et al. TAT-HSP27 Peptide Improves Neurologic Deficits via Reducing Apoptosis After Experimental Subarachnoid Hemorrhage. Front Cell Neurosci 16:878673 (2022). PubMed: 35573833
- White NM et al. Quantitative proteomic analysis reveals potential diagnostic markers and pathways involved in pathogenesis of renal cell carcinoma. Oncotarget 5:506-18 (2014). PubMed: 24504108
- Enomoto M et al. Newborn rat response to single vs. combined cGMP-dependent pulmonary vasodilators. Am J Physiol Lung Cell Mol Physiol 306:L207-15 (2014). WB . PubMed: 24242011
- Jin C et al. Plasma heat shock protein 27 is associated with coronary artery disease, abdominal aortic aneurysm and peripheral artery disease. Springerplus 3:635 (2014). PubMed: 25392804
- Thanabalasundaram G et al. Cortical non-aneurysmal subarachnoid hemorrhage post-carotid endarterectomy: a case report and literature review. Springerplus 2:571 (2013). PubMed: 24255864