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AB277428

人GOLPH2 ELISA试剂盒

Human GOLPH2 ELISA Kit

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(1 Publication)

Human GOLPH2 ELISA Kit is a Sandwich ELISA for the measurement of Human GOLPH2 in Human in Biofluids, Cell Culture Media samples.

查看别名

C9orf155, GOLPH2, PSEC0242, UNQ686/PRO1326, GOLM1, Golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2

1 Images
ELISA - Human GOLPH2 ELISA Kit (AB277428)
  • ELISA

Supplier Data

ELISA - Human GOLPH2 ELISA Kit (AB277428)

These standard curves are for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 0.5 ng/mL

范围

0.573 - 700 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

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Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)

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AB193765

Human alpha Fetoprotein ELISA Kit

Recombinant|SimpleStep

ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)

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Primary Antibodies

AB119800

Anti-GOLPH2 antibody [OTI6C9]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (AB119800)

  • 0
  • 0
Assay Kits

AB112142

MDR Assay Kit (Fluorometric)

Functional Studies - MDR Assay Kit (Fluorometric) (AB112142)

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human GOLPH2 ELISA Kit (ab277428) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GOLPH2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human GOLPH2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human GOLPH2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human GOLPH2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human GOLPH2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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回收率

[ { "sample": "Serum", "range": "80 - 146 %", "average": "= 112.3" }, { "sample": "Plasma", "range": "74 - 110 %", "average": "= 103.1" }, { "sample": "Cell culture media", "range": "124 - 144 %", "average": "= 134.2" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277428-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277428-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human GOLPH2 coated Microplate", "number":"AB277428-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277428-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277428-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277428-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human GOLPH2 Antibody", "number":"AB277428-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GOLPH2 standard protein (Lyophilized)", "number":"AB277428-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB277428-CMP04", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GOLPH2 also known as GOLM1 or Golgi membrane protein 1 has a significant mechanical role in the cell. It is a 73-kilodalton protein found mainly in the Golgi apparatus. This protein gets expressed in various tissues with notable levels in the liver and other organs involved in secretion. It acts as a transmembrane protein in the Golgi where it helps in processing and sorting secretory proteins influencing how proteins move through secretory pathways.
Biological function summary

GOLPH2 plays an essential role in maintaining Golgi function and efficient protein trafficking. It does not belong to a large protein complex but its proper functioning is critical for handling secretory pathways. The protein keeps secretory flow organized which affects cellular responses and interactions with the environment. The GOLPH2 protein also has connections to immunity responding to stimuli like infections or inflammation.

Pathways

GOLPH2 actively participates in secretory pathways and protein transport systems. Its role includes coordination with other Golgi proteins to facilitate protein modification and sorting. GOLPH2 interacts with other proteins like GOLM1 and GOLPH3 impacting the Golgi's structural organization and influencing pathways like vesicular transport. These interactions are important for adapting the intracellular environment to external demands.

GOLPH2 levels change in diseases such as liver cancer and hepatitis. These changes can serve as biomarkers for diagnosis and disease progression monitoring. GOLPH2 also connects with proteins involved in these diseases including AFP a known tumor marker. Understanding GOLPH2's role in such conditions aids research in developing diagnostic tools and therapeutic strategies.
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产品实验方案

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靶点信息

Unknown. Cellular response protein to viral infection.
See full target information GOLM1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Asia-Pacific journal of clinical oncology 20:747-753 PubMed39183450

2024

Efficacy and safety of hepatic arterial infusion chemotherapy combined with donafenib in the treatment of unresectable hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wan,Xueqin Gan,Weijie Xiong
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websiteProtocolBooklet
en
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