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重组SimpleStep

人GM-CSF ELISA试剂盒(CSF2) (ab174448)

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Other - Human GM-CSF ELISA Kit (CSF2) (ab174448)
  • Example of human GM-CSF standard curve in Sample Diluent NS.
  • Interpolated concentrations of spike GM-CSF in human serum, and plasma samples.
  • Interpolated concentrations of native GM-CSF in human cell culture supernatant sample.
  • Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.7 pg/ml
  • Range: 15.625 pg/ml - 1000 pg/ml
  • Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

概述

  • 产品名称

    人GM-CSF ELISA试剂盒(CSF2)
    参阅全部 GM-CSF 试剂盒

  • 检测方法

    Colorimetric

  • 精确度

    批次内
    样品 n Mean SD CV%
    Supernatant 8 1.4%
    批次间
    样品 n Mean SD CV%
    Supernatant 3 1.8%

  • 样品类型

    Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • 检测类型

    Sandwich (quantitative)

  • 灵敏度

    3.7 pg/ml

  • 范围

    15.625 pg/ml - 1000 pg/ml

  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 100 99% - 102%
    Serum 81 78% - 86%
    Cell culture media 100 98% - 101%
    Hep Plasma 84 84% - 86%
    EDTA Plasma 73 72% - 74%
    Cit plasma 79 77% - 80%

  • 检测时间

    1h 30m

  • 实验步骤

    One step assay

  • 种属反应性

    与反应: Human

  • 产品概述

    Human GM-CSF ELISA (ab174448) kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p53 protein in human serum, plasma and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GM-CSF with 3.7 pg/ml sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

  • 说明

    Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML).  This kit recognizes Primate and Human GM-CSF which are 95% homologous.

  • 经测试应用

    适用于: Sandwich ELISAmore details

  • 平台

    Microplate

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab174448于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Sandwich ELISA
Use at an assay dependent concentration.
说明
Sandwich ELISA
Use at an assay dependent concentration.

图片

  • Other - Human GM-CSF ELISA Kit (CSF2) (ab174448)
    Other - Human GM-CSF ELISA Kit (CSF2) (ab174448)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human GM-CSF standard curve in Sample Diluent NS.
    Example of human GM-CSF standard curve in Sample Diluent NS.

    The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spike GM-CSF in human serum, and plasma samples.
    Interpolated concentrations of spike GM-CSF in human serum, and plasma samples.

    The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated concentrations of native GM-CSF in human cell culture supernatant sample.
    Interpolated concentrations of native GM-CSF in human cell culture supernatant sample.

    The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC Cell Culture Supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 2599 pg/mL in Stimulated PBMC Cell Culture Supernatant and undetectable in unstimulated PBMC Cell Culture Supernatant.

  • Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448)
    Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448)
    To learn more about the advantages of recombinant antibodies see here.

文献 (6)

发表研究结果有使用 ab174448？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab174448 被引用在 6 文献中.

  • Selmi M  et al. Thymoquinone-loaded lipid nanocapsules with promising anticancer activity for colorectal cancer. Nanoscale Adv 5:5390-5398 (2023). PubMed: 37767034
  • Chun YY  et al. Influence of glycan structure on the colonization of Streptococcus pneumoniae on human respiratory epithelial cells. Proc Natl Acad Sci U S A 120:e2213584120 (2023). PubMed: 36943879
  • Kishton RJ  et al. Cancer genes disfavoring T cell immunity identified via integrated systems approach. Cell Rep 40:111153 (2022). PubMed: 35926468
  • Xu H  et al. HDAC4 depletion ameliorates IL-13-triggered inflammatory response and mucus production in nasal epithelial cells via activation of SIRT1/NF-κB signaling. Immun Inflamm Dis 10:e692 (2022). PubMed: 36301023
  • Watson AW  et al. Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell Rep 35:109293 (2021). PubMed: 34192535
  • Koupenova M  et al. The role of platelets in mediating a response to human influenza infection. Nat Commun 10:1780 (2019). PubMed: 30992428

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