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AB174448

人GM-CSF ELISA试剂盒(CSF2)

Human GM-CSF ELISA Kit (CSF2)

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(7 Publications)

Human GM-CSF ELISA Kit (CSF2) is a single-wash 90-min Simplestep used to quantify Human GM-CSF (CSF2) with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

查看别名

GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF

4 Images
ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)
  • ELISA

Supplier Data

ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)
Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)
  • sELISA

Unknown

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Interpolated concentrations of native GM-CSF in human cell culture supernatant sample.

The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : Stimulated PBMC Cell Culture Supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 2599 pg/mL in Stimulated PBMC Cell Culture Supernatant and undetectable in unstimulated PBMC Cell Culture Supernatant.

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)
  • sELISA

Unknown

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Interpolated concentrations of spike GM-CSF in human serum, and plasma samples.

The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)
  • sELISA

Unknown

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Example of human GM-CSF standard curve in Sample Diluent NS.

The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 3.7 pg/mL

范围

15.625 - 1000 pg/mL

检测时间

1h 30m

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human GM-CSF ELISA Kit (CSF2) ab174448 is a rapid single-wash 90-min Sandwich ELISA to measure Human GM-CSF (CSF2)in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 3.7 pg/mL.

How the assay works

Human GM-CSF (CSF2)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human GM-CSF ELISA Kit (CSF2) ab174448 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML). This kit recognizes Primate and Human GM-CSF which are 95% homologous.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "99 - 102 %", "average": "= 100" }, { "sample": "Serum", "range": "78 - 86 %", "average": "= 81" }, { "sample": "EDTA Plasma", "range": "72 - 74 %", "average": "= 73" }, { "sample": "Cell culture media", "range": "98 - 101 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "84 - 86 %", "average": "= 84" }, { "sample": "Citrate plasma", "range": "77 - 80 %", "average": "= 79" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.
Biological function summary

GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.

Pathways

GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.

GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.
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产品实验方案

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靶点信息

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
See full target information CSF2
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文献 (7)

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