AB184857

人GLP1 (7-36) ELISA试剂盒

Name: 人GLP1 (7-36) ELISA试剂盒 (ab184857)| Abcam中文官网
Brand: Abcam Limited
SKU: AB184857
Price: 8329 CNY
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Rating: 5 (3 reviews)

Human GLP1 (7-36) ELISA Kit

Recombinant
SimpleStep
了解详情

(3 Reviews)

(14 Publications)

Human GLP1 (7-36) ELISA Kit ab184857 is a single-wash 90-min SimpleStep ELISA used to quantify human glucagon-like peptide-1 (GLP-1) (7-36) with a sensitivity of 25 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450nm readout; works on any plate standard plate reader
- Different formats for different needs: -384-well format available for high-throughput applications
- Broad sample compatibility – works with plasma, cell culture supernatants, and serum
- Cited in 10 publications

查看别名

Pro-glucagon, GCG

7 Images

ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

ELISA

Supplier Data

ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

sELISA

Unknown

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Sample Data.

Ten individual healthy donors were evaluated for the presence of GLP-1 in serum using this assay. Serum of each donor was diluted 1/10 using sample diluent NS. The mean levels of GLP-1, after adjusting for dilution factor, were found at 433.5 pg/mL with a standard deviation of 277 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Example of GLP-1 standard curve. The GLP-1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Example of GLP-1 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Ten individual healthy donors were evaluated for the presence of GLP-1 in serum using this assay.

Serum of each donor was diluted 1 : 10 using sample diluent NS. The mean levels of GLP-1, after adjusting for dilution factor, were found at 433.5 pg/mL with a standard deviation of 277 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Recombinant GLP-1 (7-36) was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

sELISA

Lab

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Example of human GLP1 (7-36) standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

灵敏度

= 25 pg/mL

范围

59.6 - 1000 pg/mL

检测时间

1h 30m

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human GLP1 (7-36) ELISA Kit ab184857 is a rapid single-wash 90-min ELISA kit to measure Human GLP1 (7-36) in cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 25 pg/mL.

How the assay works

Human GLP1 (7-36) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

GLP-1(9-36) recombinant protein was diluted at 10ng/mL and assayed for cross reactivity. Signal was found at 7% from GLP-1 (7-36) protein assayed at the same concentration.

Human GLP1 (7-36) ELISA Kit ab184857 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human GLP1 (7-36) ELISA Kit ab184857

Human GLP1 (7-36) ELISA Kit ab184857 has been used to study isosinestin modulation of human glucagon like peptide 1 secretion as a therapy for diabetes mellitus⁽¹⁾ and gut microbial profiles and metabolic traits in type 1 diabetes⁽²⁾.

References:

⁽¹⁾Hyeung-Jin Jang et al. 2023, PMID: 36835092,
⁽²⁾Feihong Luo et al. 2022, PMID: 36289225

Save your precious samples

Our 384-well Human GLP1 (7-36) ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human GLP1 (7-36) Antibody Pair - BSA and Azide free ab243974
- Anti-GLP1 (7-36) antibody [EPR4042-1] - BSA and Azide free (Capture) ab244627
- Anti-GLP1 (7-36) antibody [EPR4042-407] - BSA and Azide free (Detector) ab244887

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精确度

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" } ]

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回收率

[ { "sample": "Serum", "range": "105 - 112 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "104 - 112 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "107 - 123 %", "average": "= 115" }, { "sample": "Cell culture media", "range": "93 - 102 %", "average": "= 99" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GLP-1 (7-36) also known as glucagon-like peptide-1 is a peptide hormone that is integral to glucose metabolism. It has a molecular mass of approximately 3.8 kDa and is mainly expressed in the intestinal L-cells. It also exists in neurons within the brainstem. GLP-1 (7-36) plays a role in stimulating insulin secretion from the pancreas in response to ingested nutrients. It interacts directly with the GLP-1 receptor a G protein-coupled receptor to exert its effects.

Biological function summary

The peptide regulates blood glucose levels by promoting insulin release and inhibiting glucagon secretion. GLP-1 (7-36) also slows gastric emptying and reduces appetite which contributes to its role in energy balance. It does not function as part of a larger protein complex but its active form can be derived from the proglucagon protein precursor. The peptide has additional neuroprotective effects and supports beta-cell proliferation and survival further highlighting its multifaceted roles in metabolism.

Pathways

This peptide acts significantly within the incretin pathway a critical regulator of glucose homeostasis. The GLP-1 receptor is activated upon binding with GLP-1 (7-36) leading to the stimulation of downstream signaling processes involving proteins like PKA and PI3K which promote insulin secretion. Additionally its interaction with DPP-4 enzyme is notable as DPP-4 degrades GLP-1 (7-36) diminishing its activity and influencing glucose regulation pathways.

Altered levels of GLP-1 (7-36) relate to conditions like type 2 diabetes and obesity. Its ability to enhance insulin secretion helps manage hyperglycemia in diabetic patients. The peptide also associates indirectly with liraglutide a GLP-1 analog used as a therapeutic agent in treating these conditions. The interaction between GLP-1 (7-36) and DPP-4 forms a basis for developing DPP-4 inhibitors as a treatment strategy for managing diabetes.

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产品实验方案

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靶点信息

Glucagon. Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Binds to and activates the glucagon receptor GCGR, which couples to the G(s) G protein and elevates intracellular cAMP, triggering downstream metabolic responses (PubMed : 32193322).. Glucagon-like peptide 1. Potent stimulator of glucose-dependent insulin release (PubMed : 22037645, PubMed : 40446798). Also stimulates insulin release in response to IL6 (PubMed : 22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). Has growth-promoting activities on intestinal epithelium (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744). Inhibits beta cell apoptosis (PubMed : 10605628, PubMed : 14719035, PubMed : 12554744).. Glucagon-like peptide 2. Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.. Oxyntomodulin. Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.. Glicentin. May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.

See full target information GCG

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102021 PubMed40107240

2025

Bioengineering the metabolic network of CAR T cells with GLP-1 and Urolithin A increases persistence and long-term anti-tumor activity.

Applications

Unspecified application

Species

Unspecified reactive species

Areej Akhtar,Md Shakir,Mohammad Sufyan Ansari, Divya,Md Imam Faizan,Varnit Chauhan,Aashi Singh,Ruquaiya Alam,Iqbal Azmi,Sheetal Sharma,Mehak Pracha,Insha Mohi Uddin,Uzma Bashir,Syeda Najidah Shahni,Rituparna Chaudhuri,Sarah Albogami,Rik Ganguly,Shakti Sagar,Vijay Pal Singh,Gaurav Kharya,Amit Kumar Srivastava,Ulaganathan Mabalirajan,Soumya Sinha Roy,Irfan Rahman,Tanveer Ahmad

Science advances 11:eadr5051 PubMed40009667

2025

An endogenous GLP-1 circuit engages VTA GABA neurons to regulate mesolimbic dopamine neurons and attenuate cocaine seeking.

Applications

Unspecified application

Species

Unspecified reactive species

Riley Merkel,Nicole S Hernandez,Vanessa Weir,Yafang Zhang,Antonia Caffrey,Matthew T Rich,Richard C Crist,Benjamin C Reiner,Heath D Schmidt

ACS synthetic biology 12:3433-3442 PubMed37827516

2023

Cold Exposure and Oral Delivery of GLP-1R Agonists by an Engineered Probiotic Yeast Strain Have Antiobesity Effects in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Karl Alex Hedin,Hongbin Zhang,Vibeke Kruse,Vanessa Emily Rees,Fredrik Bäckhed,Thomas U Greiner,Ruben Vazquez-Uribe,Morten Otto Alexander Sommer

International journal of molecular sciences 24: PubMed36835092

2023

Isosinensetin Stimulates Glucagon-like Peptide-1 Secretion via Activation of hTAS2R50 and the G-Mediated Signaling Pathway.

Applications

sELISA

Species

Horse

Seung-Hyeon Lee,Hyun Min Ko,Wona Jee,Hyungsuk Kim,Won-Seok Chung,Hyeung-Jin Jang

Nature communications 13:6356 PubMed36289225

2022

Functional and metabolic alterations of gut microbiota in children with new-onset type 1 diabetes.

Applications

sELISA

Species

Horse

Xiaoxiao Yuan,Ruirui Wang,Bing Han,ChengJun Sun,Ruimin Chen,Haiyan Wei,Linqi Chen,Hongwei Du,Guimei Li,Yu Yang,Xiaojuan Chen,Lanwei Cui,Zhenran Xu,Junfen Fu,Jin Wu,Wei Gu,Zhihong Chen,Xin Fang,Hongxiu Yang,Zhe Su,Jing Wu,Qiuyue Li,Miaoying Zhang,Yufeng Zhou,Lei Zhang,Guang Ji,Feihong Luo

Small (Weinheim an der Bergstrasse, Germany) 18:e2202566 PubMed36084222

2022

Sunlight-Controllable Biopharmaceutical Production for Remote Emergency Supply of Directly Injectable Therapeutic Proteins.

Applications

sELISA

Species

Horse

Bozhidar-Adrian Stefanov,Maysam Mansouri,Ghislaine Charpin-El Hamri,Martin Fussenegger

Molecular and cellular endocrinology 556:111738 PubMed35926756

2022

Sitagliptin reduces FAP-activity and increases intact FGF21 levels in patients with newly detected glucose abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Anne K N Pedersen,Camilla Hage,Niels Jessen,Linda Mellbin,Mette Bjerre

ACS synthetic biology 10:3359-3368 PubMed34842418

2021

Promoters with Consistent Expression throughout the Murine Gut.

Applications

sELISA

Species

Horse

Jeremy Armetta,Michael Schantz-Klausen,Denis Shepelin,Ruben Vazquez-Uribe,Martin Iain Bahl,Martin Frederik Laursen,Tine Rask Licht,Morten O A Sommer

International journal of molecular sciences 22: PubMed33921209

2021

NFAT5 Is Involved in GRP-Enhanced Secretion of GLP-1 by Sodium.

Applications

sELISA

Species

Horse

Caini Fan,Laureano D Asico,Van Anthony M Villar,Jessica Hunt,Santiago Cuevas,Ines Armando,Pedro A Jose,Prasad R Konkalmatt

Nutrients 12: PubMed32164195

2020

The Metabolic Syndrome: Emerging Novel Insights Regarding the Relationship between the Homeostasis Model Assessment of Insulin Resistance and other Key Predictive Markers in Young Adults of Western Algeria.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Ilyes Belhayara,Zoheir Mellouk,Mohammed Seddik Hamdaoui,Malika Bachaoui,Omar Kheroua,Willy J Malaisse

View all publications

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websiteProtocolBooklet

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人GLP1 (7-36) ELISA试剂盒

Human GLP1 (7-36) ELISA Kit

ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台