人Gelsolin ELISA试剂盒(ab270215)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 145.98 pg/ml
- Range: 546.88 pg/ml - 35000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Gelsolin ELISA试剂盒
参阅全部 Gelsolin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 2.2% 批次间 样品 n Mean SD CV% Serum 3 4% -
样品类型
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
145.98 pg/ml -
范围
546.88 pg/ml - 35000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 104 94% - 114% Hep Plasma 90 87% - 92% EDTA Plasma 94 92% - 97% Cit plasma 90 81% - 94% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Gelsolin ELISA Kit (ab270215) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Gelsolin protein in edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Gelsolin with 145.98 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Gelsolin (GSN) is an 82-kD protein with six homologous subdomains, referred to as S1-S6. Each subdomain is composed of a five-stranded β-sheet, flanked by two α-helices, one positioned perpendicular with respect to the strands and one positioned parallel. The β-sheets of the three N-terminal subdomains (S1-S3) join to form an extended β-sheet, as do the β-sheets of the C-terminal subdomains (S4-S6). It is regulated by calcium and modulates actin by binding to the plus (or barbed) ends of actin filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Gelsolin plays a role in ciliogenesis. Human Gelsolin shares 99%, 93%, 93%, and 96% sequence homology with monkey, mouse, rat, and bovine, respectively.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests 10X Human Gelsolin Capture Antibody 1 x 600µl 1 x 6000µl 10X Human Gelsolin Detector Antibody 1 x 600µl 1 x 6000µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml Antibody Diluent 5BI 1 x 6ml 10 x 6ml Human Gelsolin Lyophilized Recombinant Protein 2 vials 2 x 10 vials Plate Seal 1 unit 1 x 10 units Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units Stop Solution 1 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 1 x 120ml -
研究领域
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功能
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. -
组织特异性
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. -
疾病相关
Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure. -
序列相似性
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats. -
翻译后修饰
Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. -
细胞定位
Cytoplasm > cytoskeleton and Secreted. - Information by UniProt
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别名
- Actin depolymerizing factor
- Actin-depolymerizing factor
- ADF
see all -
数据库链接
- Entrez Gene: 2934 Human
- Omim: 137350 Human
- SwissProt: P06396 Human
- Unigene: 522373 Human
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab270215于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of human Gelsolin standard curve in Sample Diluent NS.
The Gelsolin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Gelsolin in human serum and plasma samples.The concentrations of Gelsolin were measured in duplicates, interpolated from the Gelsolin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1000, plasma (citrate) 1:1000, plasma (EDTA) 1:1000, and plasma (Heparin) 1:2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Gelsolin concentration was determined to be 12.54 µg/mL in serum, 18.80 µg/mL in plasma (citrate), 8.41 µg/mL in plasma (EDTA), and 48.98 µg/mL in plasma (Heparin).
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Sandwich ELISA - Human Gelsolin ELISA Kit (ab270215)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human Gelsolin ELISA Kit (ab270215)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Human Gelsolin ELISA Kit (ab270215)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab270215 被引用在 3 文献中.
- Jiang Y et al. GSN gene frameshift mutations in Alzheimer's disease. J Neurol Neurosurg Psychiatry 94:436-447 (2023). PubMed: 36650038
- Wang Y et al. Gelsolin: A comprehensive pan-cancer analysis of potential prognosis, diagnostic, and immune biomarkers. Front Genet 14:1093163 (2023). PubMed: 37035750
- Soares Martins T et al. Exosomal Aß-Binding Proteins Identified by "In Silico" Analysis Represent Putative Blood-Derived Biomarker Candidates for Alzheimer´s Disease. Int J Mol Sci 22:N/A (2021). PubMed: 33920336