人GC Globulin ELISA试剂盒(Vitamin D Binding蛋白) (ab108853)
Key features and details
- Sensitivity: 0.06 µg/ml
- Range: 0.098 µg/ml - 100 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
人GC Globulin ELISA试剂盒(Vitamin D Binding蛋白)
参阅全部 Vitamin D Binding protein 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6.2% 批次间 样品 n Mean SD CV% Overall 9.9% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
> 0.06 µg/ml -
范围
0.098 µg/ml - 100 µg/ml -
回收率
97 %
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检测时间
3h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human GC Globulin (Vitamin D Binding Protein) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of GC Globulin in plasma, serum, saliva, milk and cell-culture supernatants.
A GC Globulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated GC Globulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of GC Globulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 2X Biotinylated Human GC Globulin (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml GC Globulin Microplate (12 x 8 well strips) 1 unit GC Globulin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
细胞定位
Secreted. - Information by UniProt
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别名
- DBP
- DBP/GC
- GC
see all -
数据库链接
- Entrez Gene: 2638 Human
- Omim: 139200 Human
- SwissProt: P02774 Human
- Unigene: 418497 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108853于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108853.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab108853 被引用在 6 文献中.
- Lasagni Vitar RM et al. Vitamin D Supplementation Impacts Systemic Biomarkers of Collagen Degradation and Copper Metabolism in Patients With Keratoconus. Transl Vis Sci Technol 11:16 (2022). PubMed: 36580321
- Vinke P et al. Prevalence of Micronutrient Deficiencies and Relationship with Clinical and Patient-Related Outcomes in Pulmonary Hypertension Types I and IV. Nutrients 13:N/A (2021). PubMed: 34836178
- Mesinovic J et al. The Relationship between Vitamin D Metabolites and Androgens in Women with Polycystic Ovary Syndrome. Nutrients 12:N/A (2020). PubMed: 32357490
- Fernando M et al. Early Pregnancy Vitamin D Binding Protein Is Independently Associated with the Development of Gestational Diabetes: A Retrospective Cohort Study. J Clin Med 9:N/A (2020). PubMed: 32664376
- Fernando M et al. Relationships between Total, Free and Bioavailable Vitamin D and Vitamin D Binding Protein in Early Pregnancy with Neonatal Outcomes: A Retrospective Cohort Study. Nutrients 12:N/A (2020). PubMed: 32824958
- Naderpoor N et al. Bioavailable and free 25-hydroxyvitamin D and vitamin D binding protein in polycystic ovary syndrome: Relationships with obesity and insulin resistance. J Steroid Biochem Mol Biol 177:209-215 (2018). Competitive ELISA . PubMed: 28734987