人Gastrin I ELISA试剂盒(GAST) (ab133033)
Key features and details
- Sensitivity: 7.27 pg/ml
- Range: 39.1 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
人Gastrin I ELISA试剂盒(GAST)
参阅全部 Gastrin I 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Medium conc 16 471pg/ml = 3.7% High conc 16 920pg/ml = 5.7% Low conc 16 278pg/ml = 8.8% 批次间 样品 n Mean SD CV% Low conc 8 278pg/ml = 4.4% Medium conc 8 328pg/ml = 6.5% High conc 8 1065pg/ml = 3.4% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Competitive -
灵敏度
7.27 pg/ml -
范围
39.1 pg/ml - 10000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum = 101.9 % - % Tissue Culture Media = 100.6 % - % Cit plasma = 107.9 % - % -
检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Gastrin 1 (GAST) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Gastrin 1 (GAST) in tissue culture media, Human serum and Plasma (citrate).
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Gastrin 1 antigen and a polyclonal rabbit antibody specific to Gastrin 1. After incubation the excess reagents are washed away. pNpp substrate is added and catalyzed by AP to produce a yellow color. The optical density of the yellow coloration at 405 nm is inversely proportional to the amount of Gastrin 1 captured in the plate.
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说明
Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G Cells of the antrum mucosa in response to a number of stimuli associated with digestion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G Cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels will also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. Gastrin is the most potent stimulator of gastric acid secretion.
Gastrin-I (G17): H2N-Glu-Gly-Pro-Trp-Leu-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2
Gastrin is synthesized as a 101 residue pre-pro-peptide on the rough endoplasmic reticulum, then post-translationally modified by cleavage and alpha-amidation to result in the active forms G34, G17 and G13/14; Big, Little and Mini-Gastrins respectively. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine12 residue, while type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. In man, G17 has a circulating half-life of about 9 minutes while G34 has a half-life of about 35 minutes.
G34 is the major circulating gastrin in fasting serum, but with G17, increases two to three-fold after feeding until both are present in approximately equal amounts. Fasting gastrin concentration is usually less than 30 pmol/l (equivalent to 62.9 pg/ml) while patients with Zollinger-Ellison Syndrome and gastrinomas have levels exceeding 1,000 pg/ml.
Cross Reactivity
Compound % Cross Reactivity Human Gastrin I (G17-I) 100 Minigastrin (G13-I) 74.6 Rat Gastrin I 70.7 Gastrin II (G17-II, sulfated) 9.3 Cholecystokinin 26-33 (CCK-8) 2.67 Gastrin Tetrapeptide (CCK-4) 1.6 Big Gastrin (G34-I) 0.8 Gastrin Releasing Peptide (GRP) <0.001 -
经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Gastrin I Alkaline Phosphatase Conjugate 1 x 5ml Gastrin I Antibody 1 x 5ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Human Gastrin I Standard 1 x 0.5ml pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
研究领域
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相关性
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. -
细胞定位
Secreted -
别名
- Big gastrin
- G14
- G17
see all -
数据库链接
- Entrez Gene: 2520 Human
- Omim: 137250 Human
- SwissProt: P01350 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Competitive ELISA |
Use at an assay dependent concentration.
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说明 |
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Competitive ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133033 被引用在 1 文献中.
- Chang W et al. Hormonal Suppression of Stem Cells Inhibits Symmetric Cell Division and Gastric Tumorigenesis. Cell Stem Cell 26:739-754.e8 (2020). PubMed: 32142681