AB260053

人Galectin-1 ELISA试剂盒

Human Galectin-1 ELISA Kit

Recombinant
SimpleStep
了解详情

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(2 Publications)

Human Galectin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Galectin-1 with a sensitivity of 0.36 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1

4 Images

Sandwich ELISA - Human Galectin-1 ELISA Kit (AB260053)

sELISA

Supplier Data

Sandwich ELISA - Human Galectin-1 ELISA Kit (AB260053)

Human Galectin-1 standard curve comparison.

Standard Curve comparison between human RANTES SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Galectin-1 ELISA Kit (AB260053)

Interpolated concentrations of native Galectin-1 in human serum and plasma samples.

The concentrations of Galectin-1 were measured in duplicates, interpolated from the Galectin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-1 concentration was determined to be 58 ng/mL in serum, 52.5 ng/mL in plasma (EDTA) and 72.8 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Galectin-1 ELISA Kit (AB260053)

Serum from eight individual healthy human donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-1 concentration was determined to be 99.2 ng/mL with a range of 4.3 –165.5 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Galectin-1 ELISA Kit (AB260053)

Example of human Galectin-1 standard curve in Sample Diluent NS.

The Galectin-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, Heparin Plasma, Serum, EDTA Plasma

反应种属

Mouse, Rat, Cow, Human

检测类型

Sandwich (quantitative)

灵敏度

= 0.36 ng/mL

范围

0.8 - 50 ng/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Galectin-1 ELISA Kit (ab260053) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-1 protein in cell culture media, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Galectin-1 with 0.36 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Galectin-1, also known as 14 kDa lectin, galaptin, and lectin galactoside-binding soluble 1, is a β-galactoside binding lectin encoded by the gene LGALS1. Galectin-1 is a 134 amino acid non-glycosylated secreted protein that forms a non-covalently linked homodimer. Galectin-1 is expressed in a wide range of tissues and has various intra- and extra-cellular functions, where generally it is a modulator of immune responses. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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回收率

[ { "sample": "Serum", "range": "88 - 102 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "94 - 108 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "98 - 114 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "102 - 119 %", "average": "= 110" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.

Biological function summary

This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.

Pathways

The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.

Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.

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产品实验方案

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靶点信息

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69 (PubMed : 24752896).

See full target information LGALS1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular immunology 20:1339-1351 PubMed37737309

2023

Oxidized galectin-1 in SLE fails to bind the inhibitory receptor VSTM1 and increases reactive oxygen species levels in neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Jiang,Xinyue Xiao,Hao Li,Yiyi Gong,Min Wang,Huaxia Yang,Lidan Zhao,Ying Jiang,Yanping Wei,Chongchong Zhao,Jin Li,Yuling Chen,Shan Feng,Haiteng Deng,Shiliang Ma,Yue Xu,Yudong Liu,George C Tsokos,Minghong Jiang,Xuan Zhang

Biomolecules 12: PubMed36551191

2022

-GlcNAc-Mediated Regulation of Galectin Expression and Secretion in Human Promyelocytic HL-60 Cells Undergoing Neutrophilic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Adam McTague,Rada Tazhitdinova,Alexander V Timoshenko

View all publications

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websiteProtocolBooklet

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人Galectin-1 ELISA试剂盒

Human Galectin-1 ELISA Kit

Sandwich ELISA - Human Galectin-1 ELISA Kit (AB260053)

Sandwich ELISA - Human Galectin-1 ELISA Kit (AB260053)

Sandwich ELISA - Human Galectin-1 ELISA Kit (AB260053)

Sandwich ELISA - Human Galectin-1 ELISA Kit (AB260053)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台