人G-CSF ELISA试剂盒
Human G-CSF ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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Human G-CSF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human G-CSF with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin
- sELISA
Supplier Data
Sandwich ELISA - Human G-CSF ELISA Kit (AB264627)
Human G-CSF standard curve comparison.
Standard Curve comparison between human G-CSF SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human G-CSF ELISA Kit (AB264627)
Human G-CSF standard curve comparison.
Standard Curve comparison between the original human G-CSF SimpleStep ELISA kit (ab188390) and current human G-CSF SimpleStep ELISA kit (ab264627).
- sELISA
Supplier Data
Sandwich ELISA - Human G-CSF ELISA Kit (AB264627)
Interpolated concentrations of native G-CSF in human stimulated PBMC supernatant samples.
The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean G-CSF concentration was determined to be 354 pg/mL in stimulated PBMC supernatant. Unstimulated PBMC supernatant signal was undetectable.
- sELISA
Supplier Data
Sandwich ELISA - Human G-CSF ELISA Kit (AB264627)
Example of human G-CSF standard curve in Sample Diluent NS.
The G-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
反应性数据
产品详情
Human G-CSF ELISA Kit (ab264627) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of G-CSF protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human G-CSF with 11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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